Poly(I:C)

Name: Poly(I:C)
Brand: Tocris Bioscience
SKU: 4287
Rating: 5 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 4287

(2)

Citations (90)

COA / SDS

TLR3 agonist

Key Product Details

Chemical Name:

Polyinosinic-polycytidylic acid sodium salt

View Full Technical Details for Poly(I:C)

Technical Data
Citations / Reviews (2)

Biological Activity for Poly(I:C)

Poly(I:C) is a synthetic double-stranded RNA (dsRNA); endosomal Toll-like receptor 3 (TLR3) agonist. It also binds to the cytoplasmic receptors, retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated gene 5 (MDA-5), elicits the secretion of type I interferon (IFN) and pro-inflammatory cytokines. Poly(I:C) is commonly used for immune system activation in vivo. Poly(I:C) also promotes maturation of hPSC-derived cardiomyocytes by modulating early Notch signaling and cardiomyogenic transcriptional programs. Poly(I:C) promotes generation of stable, mature dendritic cells in combination with TLR8 agonist CL 075 (Cat. No. 6142). Transfection into NIT-1 β cells induces β-cell apoptosis.

To prevent denaturation, this product should be reconstituted in solutions with physiological salt concentrations.

Technical Data for Poly(I:C)

Formula	(C₁₀H₁₃N₄O₈P)_x.(C₉H₁₄N₃O₈P)_x
Storage	Store at -20°C
CAS Number	42424-50-0

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Poly(I:C)

Solubility

Soluble Soluble to 3 mg/ml in water

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References for Poly(I:C)

References are publications that support the biological activity of the product.

Y Imoto, T Takabayash, M Sakashita, Y Kato, K Yoshida, M Kidoguchi, K Koyama, N Adachi, Y Kimura, K Ogi, Y Ito, M Kanno, M Okamoto, N Narita, S Fujieda Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis Biomolecules, 2020;10(11):. 2020 PMID: 33218117
O Koenig, D Zengerle, N Perle, S Hossfeld, B Neumann, A Behring, M Avci-Adali, T Walker, C Schlensak, HP Wendel, A Nolte RNA-Eluting Surfaces for the Modulation of Gene Expression as A Novel Stent Concept Pharmaceuticals (Basel), 2017;10(1):. 2017 PMID: 28208634
Y Nagate, S Ezoe, J Fujita, D Okuzakis, D Motooka, T Ishibashi, M Ichii, A Tanimura, M Kurashige, E Morii, T Fukushima, Y Suehiro, T Yokota, H Shibayama, K Oritani, Y Kanakura Ectonucleotidase CD39 is highly expressed on ATLL cells and is responsible for their immunosuppressive function Leukemia, 2020;0(0):. 2020 PMID: 32203145
L Šenolt, K Prajzlerov, H Hulejová, B Šumová, M Filková, D Veigl, K Pavelka, J Vencovský Interleukin-20 is triggered by TLR ligands and associates with disease activity in patients with rheumatoid arthritis Cytokine, 2017;97(0):187-192. 2017 PMID: 28662439
Qiyun Zhu Duck Tembusu Virus Nonstructural Protein 1 Antagonizes IFN-? Signaling Pathways by Targeting VISA J. Immunol., 2016;0(0):. 2016 PMID: 27821666
WC Lane, MD Dunn, CL Gardner, LKM Lam, AM Watson, AL Hartman, KD Ryman, WB Klimstra The Efficacy of the Interferon Alpha/Beta Response versus Arboviruses Is Temperature Dependent MBio, 2018;9(2):. 2018 PMID: 29691338
J Latorre, JM Moreno-Nav, M Sabater, M Buxo, JI Rodriguez-, J Girones, JM Fort, R Vilallonga, W Ricart, R Simo, JM Fernandez-, FJ Ortega Decreased TLR3 in Hyperplastic Adipose Tissue, Blood and Inflamed Adipocytes is Related to Metabolic Inflammation Cell. Physiol. Biochem., 2018;51(3):1051-1068. 2018 PMID: 30476912
Sundnes O, Pietka W, Loos T, Sponheim J, Rankin A, Pflanz S, Bertelsen V, Sitek J, Hol J, Haraldsen G, Khnykin D Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences. J Invest Dermatol, 2015;135(7):1771-80. 2015 PMID: 25739051
Srivastava R, Lee S, Andrade Filho P, Lord C, Jie H, Davidson H, Lopez-Albaitero A, Gibson S, Gooding W, Ferrone S, Ferris R Cetuximab-activated natural killer and dendritic cells collaborate to trigger tumor antigen-specific T-cell immunity in head and neck cancer patients. Clin Cancer Res, 2013;19(7):1858-72. 2013 PMID: 23444227
Spranger et al. Generation of Th1-polarizing dendritic cells using the TLR7/8 agonist CL075. J.Immunol., 2010;185:738 PMID: 20511554
Robbins et al. Nuclear factor-κB translocation mediates double-stranded ribonucleic acid-induced NIT-1 beta-cell apoptosis and up-regulates caspase-12 and tumor necrosis factor-associated ligand (TRAIL). Endocrinology, 2003;144:4616 PMID: 12960048
Rouas et al. Poly(I:C) used for human dendritic cell maturation preserves their ability to secondarily secrete bioactive IL-12. Int.Immunol., 2004;16:767 PMID: 15096480
West et al. TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature, 2011;472:476 PMID: 21525932
Biermann et al. Epigenetic priming of human pluripotent stem cell-derived cardiac progenitor cells accelerates cardiomyocyte maturation. Stem Cells, 2019;37:910 PMID: 31087611