Staurosporine

Name: Staurosporine
Brand: Tocris Bioscience
SKU: 1285
Rating: 4 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 1285

(1)

Citations (111)

COA / SDS

Non-selective protein kinase inhibitor

Key Product Details

Description:	Non-selective protein kinase inhibitor
Alternative Names:	AM-2282
Chemical Name:	[9S-(9α,10β,11β,13α)]-2,3,10,11,12,13-Hexahydro-10-methoxy-9-methyl-11-(methylamino)-9,13-epoxy-1H,9H-diindolo[1,2,3-gh:3',2',1'-lm]pyrrolo[3,4-j][1,7]benzodiazonin-1-one
Purity:	≥98% (HPLC)
Molecular Weight:	466.54

View Full Technical Details for Staurosporine

Technical Data
Citations / Reviews (1)

Biological Activity for Staurosporine

Staurosporine is a broad spectrum protein kinase inhibitor. Enzymes inhibited include protein kinase C, p^60v-src tyrosine protein kinase, protein kinase A, and CaM kinase II (IC₅₀ values are 3nM, 6 nM, 7 nM and 20 nM, respectively). Staurosporine reduces nuclear myosin heavy chain 9 phosphorylation which inhibits gastric cancer cell progression in transgenic mouse models. Staurosporine inhibits cell viability and promotes apoptosis in oral and pancreatic cancer cells. Staurosporine also enhances efficiency of lentiviral transduction of human hematopoietic stem and progenitor cells by 2-fold, induces dopaminergic axonal outgrowth in vitro and triggers mitophagy. Staurosporine has high affinity (K_d = 100 nM) for the yjdF aptamer. The riboswitch function of yjdF motif RNAs is activated by Staurosporine and leads to robust reporter gene expressions in B. subtilis.

Staurosporine synthesized to Ancillary Material Grade is also available.

Scientific Data Examples for Staurosporine

	Reduced viability of differentiated cardiomyocytes exposed to cardiotoxic small molecules. The viability of differentiated cardiomyocytes was assessed using a Resazurin metabolism assay. A) Untreated cells metabolize Resazurin (Catalog # AR002) to produce resorufin, a fluorescent molecule that can be measured using a fluorometric plate reader. Resorufin fluorescence accumulated in untreated cardiomyocytes. Cells treated with the cardiotoxic small molecules Staurosporine (blue; Catalog # 1285), a non-selective protein kinase inhibitor, or Doxazosin (tan; Catalog # 2964), an alpha 1 antagonist, did not metabolize Resazurin, as shown by lack of fluorescence accumulation, indicating a loss of viability upon treatment. B) Cell morphology of untreated, Staurosporine-treated, and Doxazosin-treated cardiomyocytes was assessed by brightfield microscopy.

Technical Data for Staurosporine

M. Wt	466.54
Formula	C₂₈H₂₆N₄O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	62996-74-1
PubChem ID	44259
InChi Key	HKSZLNNOFSGOKW-FYTWVXJKSA-N
Smiles	O=C(NC6)C1=C6C4=C3C2=C1C8=C(C=CC=C8)N2[C@](C[C@@H](NC)[C@H]7OC)([H])O[C@]7(C)N3C5=CC=CC=C45

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Staurosporine

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	23.33	50

Preparing Stock Solutions for Staurosporine

The following data is based on the product molecular weight 466.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.29 mL	21.43 mL	42.87 mL
2.5 mM	0.86 mL	4.29 mL	8.57 mL
5 mM	0.43 mL	2.14 mL	4.29 mL
25 mM	0.09 mL	0.43 mL	0.86 mL

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References for Staurosporine

References are publications that support the biological activity of the product.

Wakita Staurosporine induces dopaminergic neurite outgrowth through AMP-activated protein kinase/mammalian target of rapamycin signaling pathway. Neuropharmacology 2014 PMID: 24067927
Li The yjdF riboswitch candidate regulates gene expression by binding diverse azaaromatic compounds. RNA 2016 PMID: 26843526
Zhang Effect of protein kinase C alpha, caspase-3, and survivin on apoptosis of oral cancer cells induced by staurosporine. Acta Pharmacol.Sinica 2005 PMID: 16225760
Malsy Staurosporine induces apoptosis in pancreatic carcinoma cells PaTu 8988t and Panc-1 via the intrinsic signaling pathway. Eur.J.Med.Res. 2019 PMID: 30686270
Ye Nuclear MYH9-induced CTNNB1 transcription, targeted by staurosporin, promotes gastric cancer cell anoikis resistance and metastasis. Theranostics 2020 PMID: 32685004
Lewis Staurosporine increases lentiviral vector transduction efficiency of human hematopoietic stem and progenitor cells. Mol.Ther.Methods Clin.Dev. 2018 PMID: 30038935
Yanagihara Staurosporine: an effective inhibitor for Ca²⁺/calmodulin-dependent protein kinase II. J.Neurochem. 1991 PMID: 1846174
Tamaoki Staurosporine, a potent inhibitor of phospholipid/Ca²⁺ dependent protein kinase. Biochem.Biophys.Res.Commun. 1986 PMID: 3457562
Ruegg and Burgess Staurosporine, K-252 and UCN-01: potent but nonspecific inhibitors of protein kinases. TiPS 1989 PMID: 2672462