T4

Name: T4
Brand: Tocris Bioscience
SKU: 7054

Tocris Bioscience, part of Bio-Techne | Catalog # 7054

COA / SDS

Modulator of alternative polyadenylation; inhibits rod photoreceptor gene expression via Nr2e3

Key Product Details

Description:	Modulator of alternative polyadenylation; inhibits rod photoreceptor gene expression via Nr2e3
Chemical Name:	6,7-Dimethoxy-2-[((4-phenyl-3,6-dihydropyridin-1(2H)-yl)methyl)]quinazolin-4(3H)-one
Purity:	≥98% (HPLC)
Molecular Weight:	377.44

View Full Technical Details for T4

Technical Data
Citations

Biological Activity for T4

T4 is a modulator of alternative polyadenylation (APA) (IC₅₀ values are 2.1 μM and 8.5 μM in U2OS and 293T cells, respectively). T4 promotes distal-to-proximal APA usage in multiple transcripts, using distinctive A-rich motifs through autoregulated PABPN1 signaling. T4 exhibits no inhibitory activity against a panel of 414 kinases. T4 prevents retinal degeneration in the Rho^P23H mouse model of retinitis pigmentosa. T4 inhibits rod photoreceptor gene expression via Nr2e3 in a luciferase-based assay (IC₅₀ = 0.07 μM).

Technical Data for T4

M. Wt	377.44
Formula	C₂₂H₂₃N₃O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	785708-33-0
PubChem ID	135415476
InChi Key	CKCSOMMBIPJYSG-UHFFFAOYSA-N
Smiles	COC1=C(OC)C=C2C(C(NC(CN3CCC(C4=CC=CC=C4)=CC3)=N2)=O)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for T4

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	0.75	2

Preparing Stock Solutions for T4

The following data is based on the product molecular weight 377.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.02 mM	132.47 mL	662.36 mL	1,324.71 mL
0.1 mM	26.49 mL	132.47 mL	264.94 mL
0.2 mM	13.25 mL	66.24 mL	132.47 mL
1 mM	2.65 mL	13.25 mL	26.49 mL

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References for T4

References are publications that support the biological activity of the product.

Araki Decoding transcriptome dynamics of genome-encoded polyadenylation and autoregulation with small-molecule modulators of alternative polyadenylation. Cell.Chem.Biol. 2018 PMID: 30293940
Nakamura Small molecule photoregulin3 prevents retinal degeneration in the Rho ^P23H mouse model of retinitis pigmentosa. ELife 2017 PMID: 29148976

T4