ImmPACT (TM) VIP HRP Substrate

Novus Biologicals, part of Bio-Techne | Catalog # SK-4605-NB

Novus Biologicals, part of Bio-Techne
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Key Product Details

Applications

ELISA, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Product Summary for ImmPACT (TM) VIP HRP Substrate

Features:

2-4 fold greater sensitivity than original substrate kit Optimized for IHC/ICC/ISH and blots Permanent mounting Ideal for single and multiple labeling Working solutions stable for up to 14 days at 4C One year expiry date Supplied as 120 mL of diluent and stock solutions in dropper bottles

Product Specifications

Application Notes

This kit consists of 120 mL of diluent and concentrated stock solutions of ImmPACT(TM) VIP reagents in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4C. Vector(R) VIP and ImmPACT(TM) VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted..

Kit Contents for ImmPACT (TM) VIP HRP Substrate

  • 120 ml ImmPACT™ VIP Diluent
  • 2.5 ml ImmPACT™ VIP Reagent 2
  • 2.5 ml ImmPACT™ VIP Reagent 3
  • 3 ml ImmPACT™ VIP Reagent 1
  • 3 ml ImmPACT™ VIP Reagent 4

Formulation, Preparation, and Storage

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C in the dark.

Background: ImmPACT (TM) VIP HRP Substrate

Vector(R) VIP and ImmPACT VIP HRP substrates both produce intense, violet (purple) reaction products, and can be used as alternatives to DAB or as a second color for multiple antigen labeling. Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.

Additional ImmPACT (TM) VIP HRP Substrate Products

Product Documents for ImmPACT (TM) VIP HRP Substrate

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ImmPACT (TM) VIP HRP Substrate

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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