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VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI

Novus Biologicals, part of Bio-Techne | Catalog # H-1500-NB

Novus Biologicals, part of Bio-Techne
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H-1500-NB

Key Product Details

Applications

Functional Assay, Immunocytochemistry/ Immunofluorescence, Microscopy

Product Summary for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI

VECTASHIELD Hardset Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. VECTASHIELD Hardset Mounting Medium with DAPI prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

Features:

Inhibits photobleaching of fluorescent dyes and fluorescent proteins Ideal refractive index (1.45) Ready-to-use No warming necessary Can be stored without sealing for long term analysis Hardening formulation DAPI counterstain

VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.

VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.

Product Specifications

Application Notes

Refractive index is 1.46.

NOTE: This product has a six month expiration date..

Scientific Data Images for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI

Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI [H-1500-NB]

Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI [H-1500-NB]

Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.
Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI [H-1500-NB]

Immunohistochemistry: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI [H-1500-NB]

Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label detected with Alkaline Phosphatase Anti-Fluorescein and visualized with Vector Red. Mounted in VECTASHIELD HardSet Mounting Medium
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI [H-1500-NB]

Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI [H-1500-NB]

Immunocytochemistry/Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.*

Formulation, Preparation, and Storage

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C in the dark.

Background: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI

Additional VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI Products

Product Documents for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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