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CpG oligodeoxynucleotides

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26238

Novus Biologicals, part of Bio-Techne
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NBP2-26238

Key Product Details

Species

Human

Applications

Functional Assay

Product Summary for CpG oligodeoxynucleotides

Human/Mouse Sequence CpG ODN (2216) Type A: 5' G*GGGGACGATCGTCG*G*G*G*G*G 3'

Human Sequence CpG ODN (2006) Type B: 5' T*C*G*T*C*G*T*T*T*T*G*T*C*G*T*T*T*T*G*T*C*G*T*T 3'

Human/Mouse Sequence CpG ODN (C274) Type C: 5' T*C*G*T*C*G*A*A*C*G*T*T*C*G*A*G*A*T*G*A*T 3'

Human/Mouse Sequence CpG ODN (C695) Type C: 5' T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T 3'

Human/Mouse Sequence CpG ODN (C792) Type C: 5' T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*C*G*T*T*C*G*A*A*T 3'

(* Indicates a phosphorothioate modification)

Functionality: This product is useful for the activation of TLR9 and the stimulation of TLR9 has been reported with 5-20 ug/ml.

Product Specifications

Specificity

CpG ODNs, TLR9 Ligands (human)

Applications

Ligand Activation (5-20 ug/ml)

Application Notes

A TLR9/NF-kB SEAP reporter construct in a HEK 293 cell line was used as a model system for studying hTLR9 activation.

Scientific Data Images for CpG oligodeoxynucleotides

CpG oligodeoxynucleotides [NBP2-26238]
CpG oligodeoxynucleotides [NBP2-26238] - Evaluation of various CpG ODN effects on activation of human TLR9. TLR9/NF-kB/SEAPorter HEK 293 cells were plated in 96-well plates at 7.5 x 10^4 cells/well for 16 h. Cells were treated with various CpG ODNs at different concentrations (1, 5, 10 and 20 ug/ml) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit. Note: Both negative hCpG and mCpG are the negative control ODNs.

Kit Contents for CpG oligodeoxynucleotides

  • NBP2-31134SS: 25 ug CpG Type B ODN (2006)
  • NBP2-31136SS: 25 ug CpG Type A ODN (2216)
  • NBP2-31137SS: 25 ug CpG Type C ODN (C274)
  • NBP2-31138SS: 25 ug CpG Type C ODN (C695)
  • NBP2-31139SS: 25 ug CpG Type C ODN (C792)

Formulation, Preparation, and Storage

Formulation

Sterile water

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CpG oligodeoxynucleotides

Unmethylated deoxycytosine-deoxyguanosine (CpG) dinucleotides are more frequently present in bacterial and viral DNA than in mammalian DNA1. CpG oligodeoxynucleotides (ODNs) are synthetic forms of oligonucleotides, which contains unmethylated CpG motifs, and can induce cellular immune responses through the activation of Toll-like receptor 9 (TLR9)2,3. There are three types (types A, B and C) of CpG ODNs, each of which acts differentially on stimulation of immune cells3-5. Type A CpG ODNs consist of a phosphodiester central CpG-containing palindromic motif and a phosphorothioate poly-G string at 3'-end. Type A CpG ODNs are high inducers for IFNa production in plasmacytoid dendritic cells (pDCs) but are low inducers for TLR9-dependent NF-kB induction. Type B CpG ODNs consist of a full phosphorothioate backbone with one or more CpG motifs. They are relatively higher inducers for TLR9-dependent NF-kB induction, strongly activating B cells, but are low inducers for IFNa production in pDCs. Type C CpG ODNs contain both features of Types A and B, which consist of a full phosphorothioate backbone and a CpG-containing palindromic motif. Type C CpG ODNs can induce high IFNa production in pDCs as well as B cell activation.

Alternate Names

CpG ODN, CpG ODN (2006), oligodeoxynucleotides (ODN)

Additional CpG oligodeoxynucleotides Products

Product Documents for CpG oligodeoxynucleotides

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CpG oligodeoxynucleotides

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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