Curcumin

Curcumin [NBP2-26243] - inhibition of PMA and TNF-a activated NF-kB signaling. NF-kB/SEAPorterTM HEK 293 (NBP2-26260) cells were plated in 12-well plates (0.5 x 10^6 cells/well) for 16 h. Cells were preincubated with different concentrations of DMSO-solubilized curcumin for 2 h or a DMSO vehicle (V) control. Cell were then stimulated with 10 ng/ml phorbol-12-myristate-13-acetate (PMA) [A] or 10 ng/ml TNF-a [B] for 24 h. The SEAPorter Assay Kit was used to measure SEAP, the readout assay for measuring NF-kB activation in TLR5/NF-kB cells. The results showed that the cells had basal level of NF-kB activity which was increased by PMA or TNF-a . They also show that curcumin decreased PMA and TNF-a activated NF-kB signaling in a dose-dependent manner

Ligand Activation: Curcumin [NBP2-26243]

Ligand Activation: Curcumin [NBP2-26243] - Curcumin inhibition of ligand activated TLR/NF-kB signaling. TLR5/NF-kB/SEAPorterTM HEK 293 (NBP2-26277) cells were plated in 12-well plates (0.5 x 106 cells/well) for 16 h. Cells were preincubated with increasing concentrations of DMSO-solubilized curcumin (IMG-2010) for 2 h or a DMS0 vehicle (V) control. Cell were stimulated with the TLR5 ligand Flagellin (10 ng/ml: NBP2-25289 for 24 h. The SEAPorter Assay Kit was used to measure SEAP, the readout assay for measuring NF-kB activation in TLR5/NF-kB cells. The results showed that the cells had a minimal basal level of NF-kB activity which was dramatically increased by Flagellin. They also, shown that curcumin decreased Flagellin-activated NF-kB signaling in a dose-dependent manner.

Curcumin [NBP2-26243]