EV Precipitation Solution (Blood)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11765
Product Summary for EV Precipitation Solution (Blood)
EV Precipitation Solution (Blood) is a simple and rapid method of high yield extracellular vesicle (EV) isolation from biofluids such as serum and plasma. Based on ploymer precipitation technique, samples are incubated with EV Precipitation Solution on ice and exosomes are isolated after a gentle table-top centrifugation step. EV isolation with EV Precipitation Solution is fast and requires no special equipment. Isolated EVs are suitable for a wide range of downstream analyses such as nanotracking analysis (NTA), protein profiling (western blotting, ELISA, Flow Cytometry), and nucleic acid extraction.
Product Specifications
Sample Volume
100-250 uL (plasma) or 250-500 uL (serum)
Application Notes
A 5mL solution is equivalent to 180 reactions (plasma) and 80 reactions (serum).
Scientific Data Images for EV Precipitation Solution (Blood)
EV Precipitation Solution (Blood) [NBP3-11765]
EV Precipitation Solution (Blood) [NBP3-11765] - Plasma - Exosomes isolated from plasma were resuspended in 100 ul of PBS and protein contents quantified by BCA assay. 60 ug of total protein contents were loaded with Laemmli Sample buffer 5X on acrylamide gel. WB performed using anti-ALIX antibody. Ultracentrifuged exosomes used as control (UC)Serum - Exosomes isolated from serum were resuspended in 24 ul of PBS 1X, and the entire amount was loaded with 6 ul of Laemmli Sample buffer 5X on acrylamide gel.
EV Precipitation Solution (Blood) [NBP3-11765]
EV Precipitation Solution (Blood) [NBP3-11765] - Plasma and serum: After exosome isolation resuspend pellet in 100 ul of PBS 1X and load the entire amount in a well of an ELISA plate. Example reports CD9 expression in isolated exosomes from different volumes of plasma.
EV Precipitation Solution (Blood) [NBP3-11765]
EV Precipitation Solution (Blood) [NBP3-11765] - Exosome pellet can be directly lysed with lysis buffers for nucleic acids extraction. If RNA extraction is performed by using Trizol or similar organic reagents we suggest resuspending exosomes in PBS, then to add Trizol into the mixture and to proceed with RNA purification.Formulation, Preparation, and Storage
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at room temperature.
Background: EV Precipitation Solution (Blood)
Alternate Names
EV isolation, exosome isolation, extracellular vesicle isolation
Additional EV Precipitation Solution (Blood) Products
Product Documents for EV Precipitation Solution (Blood)
Product Specific Notices for EV Precipitation Solution (Blood)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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