EV Precipitation Solution (Urine)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11766
Product Summary for EV Precipitation Solution (Urine)
EV Precipitation Solution (Urine) is a simple and rapid method of high yield extracellular vesicle (EV) isolation from urine samples. Based on polymer precipitation technique, samples are incubated with EV Precipitation Solution on ice and exosomes are isolated after a gentle table-top centrifugation step. Advantages of EV Precipitation Solution include: High yield of EVs, No prior sample concentration required, No special equipment. Isolated EVs are suitable for a wide range of downstream analyses such as nanotracking analysis (NTA), protein profiling (western blotting, ELISA, Flow Cytometry), and nucleic acid extraction.
Product Specifications
Sample Volume
5-20 mL
Application Notes
A 30mL solution is equalivant to 25 reactions (urine)
Scientific Data Images for EV Precipitation Solution (Urine)
EV Precipitation Solution (Urine) [NBP3-11766]
EV Precipitation Solution (Urine) [NBP3-11766] - Exosomes isolated from 10 ml and 20 ml of urine were resuspended in 24 ul of PBS 1X, and the entire amount was loaded with 6 ul of Laemmli Sample buffer 5X on acrylamide gel. Ultracentrifuged exosomes (30 ug) were used as control. Western blotting performed using anti-Alix (Catalog # NBP3-18364).
EV Precipitation Solution (Urine) [NBP3-11766]
EV Precipitation Solution (Urine) [NBP3-11766] - After exosome isolation resuspend pellet in 100 ul of PBS 1X and load the entire amount in a well of an ELISA plate. Example reports CD9 expression in isolated exosomes from 10 ml of urine. 30 ug of purified exosomes via ultracentrifuge (UC) were used as control.Formulation, Preparation, and Storage
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at room temperature.
Background: EV Precipitation Solution (Urine)
Alternate Names
EV isolation, exosome isolation, extracellular vesicle isolation
Additional EV Precipitation Solution (Urine) Products
Product Documents for EV Precipitation Solution (Urine)
Product Specific Notices for EV Precipitation Solution (Urine)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Loading...
Loading...
Loading...