Exosome Standards (LnCAP cell line)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11687
Key Product Details
Applications
ELISA, Flow Cytometry, Microscopy, N/A
Product Summary for Exosome Standards (LnCAP cell line)
Highly pure, lyophilized exosome standards with superior stability, optimal for multiple applications including: Assay calibration, Spike-in control for exosome quantification, Protein marker analysis for different techniques such as Western Blot and Flow Cytometry, Extraction and analysis of exosomal RNA and DNA, Standardized positive controls for evaluating immunocapture performance.
Quantity per vial of 30 ug size (number of particles in 30 ug: > 1x10^8). Quantity per vial of 100 ug size (number of particles in 100 ug: > 1x10^10). Store lyophilized exosomes up to 3 years at 4C (Expire date indicated on the label). For reconstituted exosomes store at -20C for up to one month or at -80C for up to 6 months. Avoid repeated freeze-and-thraw cycles.
Quantity per vial of 30 ug size (number of particles in 30 ug: > 1x10^8). Quantity per vial of 100 ug size (number of particles in 100 ug: > 1x10^10). Store lyophilized exosomes up to 3 years at 4C (Expire date indicated on the label). For reconstituted exosomes store at -20C for up to one month or at -80C for up to 6 months. Avoid repeated freeze-and-thraw cycles.
Scientific Data Images for Exosome Standards (LnCAP cell line)
Flow Cytometry: Exosome Standards (LnCAP cell line) [NBP3-11687]
Flow Cytometry: Exosome Standards (LnCAP cell line) [NBP3-11687] - Phenotyping assays by FACS. Reconstituted Exosomes can be used for profiling biomarkers by FACS analysis. Recommended quantity: 5 ug of reconstituted Exosomes Standards for each test.Formulation, Preparation, and Storage
Preparation Method
Isolation involves Tangential flow filtration combined with Size Exclusion Chromatogrpahy. Exosomes (small EVs) are quantified and validated for protein content and particle number by Nanoparticle Tracking Analysis as well as for common tetraspanins maker validation. Lyophilization does not alter stability of exosome proteins and nucleic acids.
Formulation
Lyophilized from cell culture media
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Reconstitution Instructions
Add deionized water, 100 ul for Standard 100 ug and 30 ul for Standard 30 ug, to get a final concentration of 1 mg/mL. Resuspend exosomes pipetting the solution up and down 10-15 times, avoiding bubbles. Vortex the reconstituted standard for 60 seconds.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C. After reconstitution store at -70C.
Background: Exosome Standards (LnCAP cell line)
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Product Documents for Exosome Standards (LnCAP cell line)
Product Specific Notices for Exosome Standards (LnCAP cell line)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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