INSTA-Blot Human Tissues
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30113
Key Product Details
Species
Human
Applications
Western Blot
Product Summary for INSTA-Blot Human Tissues
Ready-to-use PVDF membrane with 11 human tissue lysates (20 ug total protein per lane).
INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Human Tissues NBP2-30113 is a simple and fast solution for screening proteins from various human tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot͐, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
Product Specifications
Application Notes
Tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). Use in western blot reported in scientific literature (PMID: 24704276) .
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 24704276)
Scientific Data Images for INSTA-Blot Human Tissues
![Western Blot: INSTA-Blot Human Tissues [NBP2-30113]](https://resources.bio-techne.com/images/products/INSTA-Blot-Human-Tissues-SDS-Page-NBP2-30113-img0001.jpg "Western Blot: INSTA-Blot Human Tissues [NBP2-30113]")
Western Blot: INSTA-Blot Human Tissues [NBP2-30113]
Western Blot: INSTA-Blot Human Tissues [NBP2-30113] - Human Tissues. Approximately 20 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. TestisFormulation, Preparation, and Storage
Preparation Method
INSTA-Blot͐ Human Tissues NBP2-30113 contains denatured proteins from human lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution
by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot͐ is sold ready-to-use in your western blot protocol
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at room temperature.
Background: INSTA-Blot Human Tissues
Alternate Names
INSTA Blot Human Tissues, INSTA-Blot Human, INSTABlot Human Tissues
Additional INSTA-Blot Human Tissues Products
Product Documents for INSTA-Blot Human Tissues
Product Specific Notices for INSTA-Blot Human Tissues
Mark12 is used as the molecular weight marker for this product.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
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