pCLXSN Retrovirus Expression Vector
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29500
Product Specifications
Application Notes
The RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are of nonmurine origin, hence the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells) are avoided. Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture.
For amplification, transform DH-5a or similar bacteria and plate on LB plates containing 50 mg/ml ampicillin.
For amplification, transform DH-5a or similar bacteria and plate on LB plates containing 50 mg/ml ampicillin.
Scientific Data Images for pCLXSN Retrovirus Expression Vector
pCLXSN Retrovirus Expression Vector [NBP2-29500] - Schematic presentation of pCLXSN vector: CMV immediate early enhancer-promoter allows highefficiency transcription in 293 cells. However, this is lost during viral replication. Hence, the gene of interest cloned into Eco RI, Xho I, or Bam HI cloning site is driven by Moloney MLV and murine sarcoma virus LTR (RU5).
Formulation, Preparation, and Storage
Formulation
10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: pCLXSN Retrovirus Expression Vector
Alternate Names
pCLXSN, pCLXSN Retrovirus, Retrovirus, Retrovirus Expression Vector
Additional pCLXSN Retrovirus Expression Vector Products
Product Documents for pCLXSN Retrovirus Expression Vector
Product Specific Notices for pCLXSN Retrovirus Expression Vector
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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