Product Overview
The RNAscope Plus smRNA-RNA Fluorescent Assays use a proprietary method of in situ hybridization to simultaneously visualize one 17-50 nucleotide smRNA target, microRNA (miRNAs), PIWI-interacting RNA (piRNAs), short interfering RNA (siRNAs), or antisense oligonucleotides (ASOs)) simultaneously with three RNA targets. The assay is available for manual or automated use.
Product Features
Two Chemistries in One Workflow
Two Chemistries in One Workflow
•Detect one small RNA using miRNAscope chemistry
•Detect up to 3 RNAs using RNAscope chemistry
Fluorescent Multiplex Detection
Fluorescent Multiplex Detection
•Visualize up to 4 targets
•Utilize Tyramide Signal Amplification (TSA) system for increased signal to noise ratio
Flexible Assay Design
Flexible Assay Design
•Automated or manual workflows available
•Choose any miRNAscope probe and up to any 3 RNAscope probes
•Open fluorophore assignment
RNAscope Plus smRNA-RNA assays allow simultaneous detection of 1 smRNA and up to 3 RNA targets within a single sample using up to four target probes and the nuclei can be counterstained and visualized using DAPI fluorescent dye. The four target probes, are each designed in a different probe channel—S1 for smRNA, and C2, C3, and/or C4 for RNA targets. The assay is compatible with formalin-fixed, paraffin-embedded (FFPE) and fresh frozen (FF) tissue.
The key technological advantage of this assay is the significant signal boost with simultaneous background noise suppression which is due to advances in ACD’s patented signal amplification technology combined with Tyramide Signal Amplification (TSA™) technology.
Watch Our Short Introductory Video
Learn about the RNAscope Plus smRNA-RNA assay and see assay data detecting miRNAs in tumor and in brain, as well as data detecting siRNA for therapeutic studies.
Featured Applications
Neuronal Disease and Development
Neuronal Disease and Development
The multiplexing capability for the simultaneous detection of multiple neuronal cell markers allows robust gene expression analysis and visualization of distinct cell populations within the nervous system.
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Find probes below or request a custom design if you don’t see your target..
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| ACD Components | # of Slides | Catalog # |
|---|---|---|
| Consumables from Leica Biosystems | Catalog # | |
RNAscope™ Plus smRNA-RNA LS Reagents Kit
| 60 | 322786 |
| miRNAscope LS Target Probes (Catalog or Made-to-Order LS-S1 Probes) | 30 | Various |
| RNAscope LS 2.5 Target Probes (Catalog or Made-to-Order C2, C3 & C4 LS 2.5 Probes) | 30 | Various |
| RNAscope Plus smRNA-RNA 4-plex LS Positive Control Probe-Hs | 30 | 323368 |
| RNAscope Plus smRNA-RNA 4-plex LS Positive Control Probe-Mm | 30 | 323378 |
| RNAscope Plus smRNA-RNA 4-plex LS Positive Control Probe-Rn | 30 | 323388 |
| RNAscope Plus smRNA-RNA 4-plex LS Negative Control Probe | 30 | 323398 |
| TSA Vivid™ 520 nm | See Channel Selection guide for options>> | 323271 |
| TSA Vivid™ 570 nm | 323272 | |
| TSA Vivid™ 650 nm | 323273 | |
| Consumables from Akoya Biosciences | Catalog # | |
| Opal 520 Reagent Pack | See Channel Selection guide for options>>
| FP1487001KT |
| Opal 570 Reagent Pack | FP1488001KT | |
| Opal 620 Reagent Pack | FP1495001KT | |
| Opal 650 Reagent Pack | FP1496001KT | |
| Opal 690 Reagent Pack | FP1497001KT | |
Opal Polaris 780 Reagent Pack Antibody Diluent/Block (if Opal Polaris 780 is used)
| FP1501001KT ARD1001EA | |
| BOND 30 mL Open containers | OP309700 | |
| BOND 6 mL Titration kit | OPT9049 | |
| BOND Research Detection System (2) | DS9455/DS9777 | |
| BOND Universal Covertile | S21.4611/S21.2001 | |
| BOND Epitope Retrieval Solution 1-1L (RTU) | AR9961 | |
| BOND Epitope Retrieval Solution 2-1L (RTU) | AR9640 | |
| BOND Dewax Solution – 1L (RTU) | AR9222 | |
| BOND Wash Solution 10X Concentrate – 1L | AR9590 | |
| BOND Aspirating Probe Cleaning System | CS9100 | |
| BOND Mixing Stations | S21.1971 | |
Prefer the bench? Run up to 20 slides at a time with our manual assay.
| ACD Components | # of Slides | Catalog # |
|---|---|---|
20 | 322785 | |
miRNAscope Target Probes (Catalog or Made-to-Order LS-S1 Probes) | 20 | Various |
RNAscope 2.5 Target Probes | 20 | Various |
RNAscope Plus smRNA-RNA 4-plex Positive Control Probe-Hs | 20 | 323361 |
RNAscope Plus smRNA-RNA 4-plex Positive Control Probe-Mm | 20 | 323371 |
RNAscope Plus smRNA-RNA 4-plex Positive Control Probe-Rn | 20 | 323381 |
RNAscope Plus smRNA-RNA 4-plex Negative Control Probe | 20 | 323391 |
323271 | ||
323272 | ||
323273 | ||
variable | 310018 | |
HybEZ™ Hybridization System | Learn about the HybEZ II hybridization System>> | |
| Consumables from Akoya Biosciences | Catalog # | |
Opal 520 Reagent Pack | See Channel Selection guide for options>> | FP1487001KT |
Opal 570 Reagent Pack | FP1488001KT | |
Opal 620 Reagent Pack | FP1495001KT | |
Opal 650 Reagent Pack | FP1496001KT | |
Opal 690 Reagent Pack | FP1497001KT | |
Opal Polaris 780 Reagent Pack Antibody Diluent/Block (if Opal Polaris 780 is used) | FP1501001KT ARD1001EA | |
RNAscope Plus smRNA-RNA LS assay supports interrogation of up to 4 target probes. The assay uses four target probes with each probe designed in a different channel – the S1 channel for smRNA and C2, C3 and/or C4 channels for other RNA targets. The nuclei can be counterstained and visualized using DAPI fluorescent dye. For viewing of 4 plex assay, a multiplexed biomarker imaging system is required, such as Nuance FX Multiplex Biomarker Imaging System, Vectra 3.0 Automated Quantitative Pathology Imaging System or Mantra Quantitative Pathology Workstation Akoya Biosciences.
Fluorophore options depend on the imaging systems available. Two-color combination examples are shown in the following table. Multispectral un-mixing capability are required for certain options. Recommended dilution ranges are shown in ().
Microscopy Channel | Fluorophores Option 1 | Fluorophores Option 2 |
S1 | Opal™ 690nm (1:750–1:3000) | TSA Vivid™ 650nm (1:750–1:3000) |
C2 | Opal™ 520nm (1:750–1:3000) | TSA Vivid™ 520nm (1:750–1:3000) |
C3 | Opal™ 570nm (1:750–1:3000) | TSA Vivid™ 570nm (1:750–1:3000) |
C4 | Opal™ 780nm (TSA-DIG: 1:750–1:3000) (Polaris 780: 1:187.5–1:750 ) | Opal™ 780nm (TSA-DIG: 1:750–1:3000) (Polaris 780: 1:187.5–1:750 ) |