Novel Fully Automated Multiomics Assay for Profiling the Immune Cell Landscape and Activation States

The immune system plays a critical role in combating several types of malignancies. Immunotherapies including checkpoint blockade therapies have utilized the innate ability of the  immune system to identify and eliminate malignant cells. It has also been established that the profile of tumor-infiltrating lymphocytes (TILs) can to some extent predict patient response and overall survival. Traditionally, standard techniques such as flow cytometry and immunofluorescence have enabled successful assessment of tumor immune profile but identifying information about their activation states and cytotoxic effects has been challenging. Spatial technologies that enable the simultaneous detection of cytokines and  chemokines with immune cell markers, can provide information about the immune cell composition and enable a comprehensive understanding of mechanisms underlying immune recruitment, infiltration and exclusion.

• The HiPlex Pro assay on COMET was able to detect key cytokine markers within the tumor-immune landscape in melanoma tissue.
• The single-cell resolution of the HiPlex Pro workflow on COMET enables robust immune cell profiling to identify sub-types implicated in disease initiation and progression.
• The multiomics workflow was successfully able to demonstrate the detection of chemokines involved in T cell and dendritic cell recruitment which is crucial for activation of the anti-tumor adaptive immune response.