Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated and backed by Cell Signaling Technology (CST)
Simple Western Validated Antibodies
Filter Antibodies: (5711)
| Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Citations & Supporting Data | Host Species | Sample | Separation | Dilution | kDa on Simple Western |
|---|---|---|---|---|---|---|---|---|---|
| 14-3-3 β | Primary |
Invitrogen Antibodies PA1-37002 |
14-3-3 protein beta/alpha |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:1000 | |
| 14-3-3 β/α | Primary |
Cell Signaling Technology 9636 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 ε | Primary |
Cell Signaling Technology 9635 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 η | Primary |
Cell Signaling Technology 9640 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 γ | Primary |
Novus Biologicals NB100-406 |
Unmodified | Datasheet | Mouse | HeLa lysate 1.0 mg/mL | Size | 1:12.5 | 36 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27202 |
Unmodified | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:50 | 33 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27370 |
Unmodified | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:10 | 34 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-81016 |
Datasheet | Mouse | 1:12.5 | ||||
| 14-3-3 γ | Primary |
Cell Signaling Technology 9637 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 γ | Primary |
Santa Cruz Biotechnology sc-731 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Size | 1:100 | 39 | |
| 14-3-3 τ | Primary |
Cell Signaling Technology 9638 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 ζ | Primary |
R&D Systems MAB2669 |
Unmodified | Datasheet | Mouse | SH-SY5Y human neuroblastoma cell line | Size | 2 µg/mL | 34 |
| 14-3-3 ζ/δ | Primary |
Cell Signaling Technology 9639 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 (pan) | Primary |
Cell Signaling Technology 8312 |
Pan |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 15-PGDH/HPGD | Primary |
R&D Systems AF5660 |
Unmodified | Datasheet | Goat | Human liver tissue | Size | 10 µg/mL | 34 |
| 15-PGDH/HPGD | Primary |
Novus Biologicals NB200-179 |
Unmodified | Datasheet | Rabbit | Lovo lysate 0.5 mg/mL | Size | 1:500 | 34 |
| 15-PGDH/HPGD* | Primary |
Cell Signaling Technology 94019 |
Datasheet | Rabbit | RT-4 | Size | 1:50–1:250 | 33 | |
| 17 β-HSD1/HSD17B1 | Primary |
R&D Systems MAB7178 |
Unmodified | Datasheet | Mouse | Human placenta tissue | Size | 2.5 µg/mL | 40 |
| 20S Proteasome α3 | Primary |
Santa Cruz Biotechnology sc-166205 |
Unmodified |
Citations
(1) Datasheet |
Mouse | Bone marrow | Size | 1:100 | 33 |
| 3β-HSD | Primary |
Santa Cruz Biotechnology sc-100466 |
Unmodified |
Citations
(1) Datasheet |
Mouse | Kidneys | Size | 1:50 | 42 |
| 3β-HSD | Primary |
Santa Cruz Biotechnology sc-28206 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Testes | Size | 1:100 | 42 |
| 4-1BB/TNFRSF9 | Primary |
R&D Systems AF838 |
Unmodified | Datasheet | Goat | HDLM2 human Hodgkin’s lymphoma cells | Size | 1:20 | 58 |
| 4-1BB/TNFRSF9 | Primary |
R&D Systems MAB8381 |
Unmodified | Datasheet | Mouse | HDLM2 human Hodgkin’s lymphoma cells | Size | 1:20 | 49, 57 |
| 4-Hydroxynonenal | Primary |
R&D Systems MAB3249 |
Citations
(1) Datasheet |
Mouse | HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) | Size | 5 µg/mL | ||
| 4-Hydroxynonenal (HNE) | Primary |
Cell Biolabs STA-035 |
Citations
(1) Datasheet |
Rabbit | Size | ||||
| 4E-BP1 | Primary |
R&D Systems AF3227 |
Eukaryotic translation initiation factor 4E-binding protein 1 | Datasheet | Goat | HeLa human cervical epithelial carcinoma cell line | Size | 5 µg/mL | 26 |
| 4E-BP1 | Primary |
Cell Signaling Technology 4923 |
Eukaryotic translation initiation factor 4E-binding protein 1 | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50–1:200 | ||
| 4E-BP1 | Primary |
Epitomics 1557-1 |
Eukaryotic translation initiation factor 4E-binding protein 1 | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50–1:200 | ||
| 4E-BP1 | Primary |
Cell Signaling Technology 9452 |
Eukaryotic translation initiation factor 4E-binding protein 1 |
Citations
(8) Datasheet |
Rabbit | Muscle | Size | 1:50 | |
| 4E-BP1 | Primary |
MilliporeSigma 07-1416 |
Eukaryotic translation initiation factor 4E-binding protein 1 | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50–1:200 | ||
| 4E-BP1* | Primary |
Cell Signaling Technology 9644 |
Eukaryotic translation initiation factor 4E-binding protein 1 |
Citations
(5) Datasheet |
Rabbit | HepG2 | Size | 1:50 – 1:250 | 24 |
| 4E-BP2 | Primary |
Cell Signaling Technology 2845 |
Eukaryotic translation initiation factor 4E-binding protein 2 |
Citations
(1) Datasheet |
Rabbit | SH-SY5Y | Size, Charge | ||
| 4F2hc/CD98 (D6O3P) | Primary |
Cell Signaling Technology 13180 |
Citations
(1) Datasheet |
Rabbit | HEK293T, MDA-MB-231, PANC-1 | Size | 1:250 | ||
| 4HPPD | Primary |
Proteintech 17004-1-AP |
Rabbit | recombinant protein expression in E. coli | Size | 1:50 | 52 | ||
| 5'-Nucleotidase/CD73 | Primary |
R&D Systems AF5795 |
Datasheet | Sheep | U266 human myeloma cell line | Size | 10 µg/mL | 86 | |
| 5-HT1A | Primary |
Novus Biologicals NBP2-21590 |
Citations
(4) Datasheet |
Rabbit | |||||
| 5-HT1D | Primary |
Novus Biologicals NB100-56349 |
Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:10 | ||
| 5-HT2AR | Primary |
LSBio LS-C172270 |
Citations
(2) Datasheet |
Rabbit | Rat cortex | Size | 1:500 | ||
| 5-HT3A | Primary |
Novus Biologicals NB100-56351 |
Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:50 | 65 | |
| 5-HT7 | Primary |
Novus Biologicals NB100-56352 |
5-hydroxytryptamine receptor 7 |
Citations
(5) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 5-Lipoxygenase | Primary |
Novus Biologicals NB110-58748 |
Datasheet | Rabbit | Human Lung lysate 0.5 mg/mL | Size | 50 µg/mL | ||
| 5-Lipoxygenase | Primary |
Novus Biologicals NB110-58749 |
Datasheet | Rabbit | Human Lung lysate 0.5 mg/mL | Size | 10 µg/mL | ||
| 53BP1 | Primary |
Abcam ab172580 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Skin | Size | 1:50 | |
| 53BP1 (phospho S25) | Primary |
Abcam ab70323 |
Phospho,Ser25 |
Citations
(1) Datasheet |
Rabbit | Skin | Size | 1:50 | |
| 5T4 | Primary |
R&D Systems AF4975 |
Datasheet | Sheep | Human placenta | Size | 50 µg/mL | 90 | |
| 6x-His Tag | Primary |
Novus Biologicals NB100-64768 |
Epitope tag | Datasheet | Mouse | rhEDG6 CHO S Tx lysate 0.1 mg/mL, Recombinant EGFP (left) and rhSNAP25-GFP (right) lysate 1.25 mg/mL | Size | 1:250, 1:50 | |
| 6x-His Tag | Primary |
Abcam ab18184 |
Epitope tag |
Citations
(2) Datasheet |
Mouse | Size | |||
| 6x-His Tag | Primary |
Takara Bio 631212 |
Epitope tag |
Citations
(1) Datasheet |
Mouse | Size | 1:50 | ||
| 6x-His Tag | Primary |
Invitrogen Antibodies 710286 |
Epitope tag |
Citations
(1) Datasheet |
Rabbit | Blood | Size | 55 | |
| 6x-His Tag | Primary |
Invitrogen Antibodies PA1-983B-HRP |
Epitope tag |
Citations
(1) Datasheet |
Rabbit | Blood | Size | 55 |