Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated and backed by Cell Signaling Technology (CST)
Simple Western Validated Antibodies
Filter Antibodies: (6776)
| Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Citations & Supporting Data | Host Species | Sample | Separation | Dilution | kDa on Simple Western |
|---|---|---|---|---|---|---|---|---|---|
| α-Actinin-4 | Primary |
GeneTex GTX101669 |
alpha (α) Actinin 4, RRID:AB_10617403 |
Citations
(1) Datasheet |
Rabbit | UC-sEVs and SEC-sEVs | Size | 32 µg/mL | ~100 kDa |
| α-Elastin | Primary |
Invitrogen Antibodies PA5-72440 |
Elastin, RRID:AB_2718294 |
Citations
(1) Datasheet |
Rabbit | 1:20 | |||
| α-Sarcomeric actin | Primary |
MilliporeSigma A2172 |
Purified rabbit striated muscle actin used as immunogen, RRID:AB_476695 |
Citations
(1) Datasheet |
Mouse | Protein lysates | Size | 1:10,000 | |
| α-SMA | Primary |
Abcam ab21027 |
alpha (α) smooth muscle Actin, RRID:AB_1951138 |
Citations
(1) Datasheet |
Goat | Lung fibroblasts | 1:50 | ||
| α-SMA | Primary |
Invitrogen Antibodies 14-9760-82 |
alpha (α)-Smooth Muscle Actin, RRID:AB_2572996 |
Citations
(1) Datasheet |
Mouse | Fibroblasts | 1:100 | ||
| α-SMA | Primary |
Dako M0851 |
alpha (α) SMA, RRID:AB_2223500 |
Citations
(1) Datasheet |
Mouse | Rabbit corneal fibroblasts treated with TGF beta | Size | 1:100 | 48 |
| α-Synuclein | Primary |
Cell Signaling Technology 2642 |
alpha (α)-Synuclein , RRID:AB_2192679 |
Citations
(1) Datasheet |
Rabbit | Size | 1:25 | 21 | |
| α-Tubulin | Primary |
Abcam ab52866 |
alpha (α) Tubulin [EP1332Y], RRID:AB_869989 |
Citations
(1) Datasheet |
Rabbit | Cytoplasmic Fraction | Size | 2.7 μg/mL | 54 |
| α-tubulin | Primary |
Invitrogen Antibodies PA5-16891 |
alpha (α) Tubulin, RRID:AB_10984790 |
Citations
(1) Datasheet |
Rabbit | 1:1 | |||
| α7nAChR | Primary |
Abcam ab216485 |
Citations
(1) Datasheet |
Rabbit | Airway Smooth Muscle (ASM) | 1:50 | |||
| β-actin | Primary |
Bioss bs-0061R |
beta (β) Actin, RRID:AB_10855480 |
Citations
(1) Datasheet |
Rabbit | Rat kidney, pancreas, and heart | Size | 1:25, 1:75, 1:100, 1:150, 1:300 | 48 |
| β-Actin | Primary |
Proteintech 20536-1-AP |
beta (β) Actin , RRID:AB_10700003 |
Citations
(3) Datasheet |
Rabbit | Protein extract from tissues | 1:200 | ||
| β-actin | Primary |
Cell Signaling Technology 5125 |
beta (β) Actin , RRID:AB_1903890 |
Citations
(2) Datasheet |
Rabbit | Frozen samples | 1:2000 | ||
| β-Actin | Primary |
Abcam ab213262 |
beta (β) Actin , RRID:AB_2924820 |
Citations
(1) Datasheet |
Rabbit | Liver tissue homogenate (mouse) | |||
| β-Actin | Primary |
Wanleibio WL01372 |
beta (β) Actin , RRID:AB_2847841 |
Citations
(1) Datasheet |
Rabbit | Cell lysates | |||
| β-actin | Primary |
PTM Bio PTM-5018 |
beta (β) Actin, RRID:AB_3086678 |
Citations
(1) Datasheet |
Mouse | Retinal tissue | Size | 1:50 | |
| β-Actin | Primary |
Proteintech 81115-1-RR |
beta (β) Actin, RRID:AB_2923704 |
Citations
(1) Datasheet |
Rabbit | Supernatant from lysate | |||
| β-actin | Primary |
ABclonal AC026 |
ACTB, RRID:AB_2768234 |
Citations
(2) Datasheet |
Rabbit | Control tissue samples | |||
| β-actin | Primary |
Abcam ab179467 |
actin, RRID:AB_2737344 |
Citations
(1) Datasheet |
Rabbit | Cell lysates | |||
| β-Actin | Primary |
ABclonal AC004 |
beta (β) Actin , RRID:AB_2737399 |
Citations
(1) Datasheet |
Mouse | Phf2 KO myoblasts | 1:50 | ||
| β-Actin | Primary |
Abcam ab115777 |
beta (β) Actin antibody [SP124], RRID:AB_10899528 |
Citations
(1) Datasheet |
Rabbit | Vascular smooth muscle cells (VSMCs) | 1:150 | ||
| β-actin | Primary |
Abcam ab124964 |
alpha (α) smooth muscle Actin [EPR5368], RRID:AB_11129103 |
Citations
(2) Datasheet |
Rabbit | Human Umbilical Vein Endothelial Cells (HUVECs) | 1:400 | ||
| β-actin (Mouse monoclonal clone [AC-15]) | Primary |
Abcam ab49900 |
beta (β) Actin , RRID:AB_867494 |
Citations
(1) Datasheet |
Mouse | 1:100 | |||
| β-amyloid | Primary |
Santa Cruz Biotechnology sc-28365 |
beta (β) Amyloid, RRID:AB_626669 |
Citations
(2) Datasheet |
Mouse | Fibroblasts, Mouse brain | Size | 1:50 | |
| β-catenin | Primary |
ZenBio 201328-5D6 |
Citations
(1) Datasheet |
Mouse | Intestinal tissue | ||||
| β-Catenin | Primary |
Cell Signaling Technology 2951 |
Catenin, beta, RRID:AB_1196644 |
Citations
(1) Datasheet |
Total protein extracted (KYSE70 cells) | 1:50 | |||
| β-catenin | Primary |
Santa Cruz Biotechnology sc-7963 |
Raised against amino acids 680-781 mapping at the C-terminus of human b-Cat, RRID:AB_626807 |
Citations
(1) Datasheet |
Mouse | Lung cancer cell | Size | 1:50 | |
| β-Tubulin | Primary |
MilliporeSigma T8328 |
RRID:AB_1844090 |
Citations
(1) Datasheet |
Mouse | Size | |||
| γ-ACTIN | Primary |
GeneTex GTX101794 |
Actin γ 1, RRID:AB_2036153 |
Citations
(1) Datasheet |
Rabbit | Human Stomach Smooth Muscles | Size | 1:100 | 44 |
| γ-Tubulin | Primary |
MilliporeSigma T6557 |
Tubulin, gamma, RRID:AB_477584 |
Citations
(2) Datasheet |
Mouse | Cell lysates | 8 µg/mL | ||
| 1-acyl-sn-glycerol-3-phosphate acyltransferase beta | Primary |
MilliporeSigma HPA019544 |
1-acyl-sn-glycerol-3-phosphate acyltransferase beta, RRID:AB_1844646 |
Citations
(1) Datasheet |
Rabbit Polyclonal | Placenta | Size | 1:100 | |
| 14-3-3 β | Primary |
Invitrogen Antibodies PA1-37002 |
14-3-3 protein beta (β)/alpha (α), RRID:AB_2217649 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:1000 | |
| 14-3-3 β/α | Primary |
Cell Signaling Technology 9636 |
Unmodified, RRID:AB_560823 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 ε | Primary |
Cell Signaling Technology 9635 |
Unmodified, RRID:AB_2217758 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 η | Primary |
Cell Signaling Technology 9640 |
Unmodified, RRID:AB_2218073 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 γ | Primary |
Novus Biologicals NB100-406 |
Unmodified, RRID:AB_10002615 | Datasheet | Mouse | HeLa lysate 1.0 mg/mL | Size | 1:12.5 | 36 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27202 |
Unmodified, RRID:AB_3275422 | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:50 | 33 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27370 |
Unmodified, RRID:AB_3276333 | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:10 | 34 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-81016 |
RRID:AB_3410086 | Datasheet | Mouse | 1:12.5 | |||
| 14-3-3 γ | Primary |
Cell Signaling Technology 9637 |
Unmodified, RRID:AB_2218254 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 γ | Primary |
Santa Cruz Biotechnology sc-731 |
Unmodified, RRID:AB_2217962 |
Citations
(1) Datasheet |
Rabbit | Size | 1:100 | 39 | |
| 14-3-3 τ | Primary |
Cell Signaling Technology 9638 |
Unmodified, RRID:AB_2218251 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 ζ | Primary |
R&D Systems MAB2669 |
Unmodified, RRID:AB_2814945 | Datasheet | Mouse | SH-SY5Y human neuroblastoma cell line | Size | 2 µg/mL | 34 |
| 14-3-3 ζ/δ | Primary |
Cell Signaling Technology 9639 |
Unmodified, RRID:AB_2273196 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 (pan) | Primary |
Cell Signaling Technology 8312 |
Pan, RRID:AB_10860606 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 15-PGDH/HPGD | Primary |
R&D Systems AF5660 |
Unmodified, RRID:AB_2120408 | Datasheet | Goat | Human liver tissue | Size | 10 µg/mL | 34 |
| 15-PGDH/HPGD | Primary |
Novus Biologicals NB200-179 |
Unmodified, RRID:AB_10002546 | Datasheet | Rabbit | Lovo lysate 0.5 mg/mL | Size | 1:500 | 34 |
| 15-PGDH/HPGD* | Primary |
Cell Signaling Technology 94019 |
Datasheet | Rabbit | RT-4 | Size | 1:50–1:250 | 33 | |
| 17 β-HSD1/HSD17B1 | Primary |
R&D Systems MAB7178 |
Unmodified, RRID:AB_3658719 | Datasheet | Mouse | Human placenta tissue | Size | 2.5 µg/mL | 40 |
| 20S Proteasome α3 | Primary |
Santa Cruz Biotechnology sc-166205 |
Unmodified, RRID:AB_2253046 |
Citations
(1) Datasheet |
Mouse | Bone marrow | Size | 1:100 | 33 |
| 26S proteasome non-ATPase regulatory subunit 8 | Primary |
Santa Cruz Biotechnology sc-514053 |
Citations
(1) Datasheet |
Mouse | HRTPT | Size | 1:50 | Not stated | |
| 3β-HSD | Primary |
Santa Cruz Biotechnology sc-100466 |
Unmodified, RRID:AB_1118475 |
Citations
(1) Datasheet |
Mouse | Kidneys | Size | 1:50 | 42 |
| 3β-HSD | Primary |
Santa Cruz Biotechnology sc-28206 |
Unmodified, RRID:AB_2120105 |
Citations
(1) Datasheet |
Rabbit | Testes | Size | 1:100 | 42 |
| 3-hydroxyanthranilate 3,4-dioxygenase | Primary |
Proteintech 12791-1-AP |
3-hydroxyanthranilate 3,4-dioxygenase, RRID:AB_2115432 |
Citations
(1) Datasheet |
Rabbit | Liver | Size | 1:300 | |
| 4-1BB/TNFRSF9 | Primary |
R&D Systems AF838 |
Unmodified, RRID:AB_355648 | Datasheet | Goat | HDLM2 human Hodgkin’s lymphoma cells | Size | 1:20 | 58 |
| 4-1BB/TNFRSF9 | Primary |
R&D Systems MAB8381 |
Unmodified, RRID:AB_3659150 | Datasheet | Mouse | HDLM2 human Hodgkin’s lymphoma cells | Size | 1:20 | 49, 57 |
| 4-Hydroxynonenal | Primary |
R&D Systems MAB3249 |
RRID:AB_664165 |
Citations
(1) Datasheet |
Mouse | HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) | Size | 5 µg/mL | |
| 4-Hydroxynonenal (4HNE) | Primary |
Abcam ab46545 |
HNE conjugated to BC., RRID:AB_722490 |
Citations
(1) Datasheet |
Rabbit | Redox EVs | 1:25 | ||
| 4-Hydroxynonenal (HNE) | Primary |
Cell Biolabs STA-035 |
Citations
(1) Datasheet |
Rabbit | Size | ||||
| 4E-BP1 | Primary |
R&D Systems AF3227 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_2097980 | Datasheet | Goat | HeLa human cervical epithelial carcinoma cell line | Size | 5 µg/mL | 26 |
| 4E-BP1 | Primary |
Cell Signaling Technology 4923 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_659944 | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50–1:200 | ||
| 4E-BP1 | Primary |
Epitomics 1557-1 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_562030 | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50–1:200 | ||
| 4E-BP1 | Primary |
MilliporeSigma 07-1416 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_1586861 | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50–1:200 | ||
| 4E-BP1 | Primary |
Cell Signaling Technology 9452 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_331692 |
Citations
(14) Datasheet |
Rabbit | Muscle | Size | 1:50 | |
| 4E-BP1* | Primary |
Cell Signaling Technology 9644 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_2097841 |
Citations
(6) Datasheet |
Rabbit | HepG2 | Size | 1:50 – 1:250 | 24 |
| 4E-BP2 | Primary |
Cell Signaling Technology 2845 |
Eukaryotic translation initiation factor 4E-binding protein 2, RRID:AB_10699019 |
Citations
(1) Datasheet |
Rabbit | SH-SY5Y | Size, Charge | ||
| 4F2hc/CD98 (D6O3P) | Primary |
Cell Signaling Technology 13180 |
4F2hc/CD98, RRID:AB_2687475 |
Citations
(1) Datasheet |
Rabbit | HEK293T, MDA-MB-231, PANC-1 | Size | 1:250 | |
| 4HPPD | Primary |
Proteintech 17004-1-AP |
RRID:AB_2264310 | Rabbit | recombinant protein expression in E. coli | Size | 1:50 | 52 | |
| 5'-Nucleotidase/CD73 | Primary |
R&D Systems AF5795 |
RRID:AB_1964487 | Datasheet | Sheep | U266 human myeloma cell line | Size | 10 µg/mL | 86 |
| 5-HT1A | Primary |
Novus Biologicals NBP2-21590 |
RRID:AB_3102030 |
Citations
(5) Datasheet |
Rabbit | ||||
| 5-HT1D | Primary |
Novus Biologicals NB100-56349 |
RRID:AB_838864 | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:10 | |
| 5-HT2AR | Primary |
LSBio LS-C172270 |
HTR2A / 5‑HT2A Receptor, RRID:AB_3102031 |
Citations
(2) Datasheet |
Rabbit | Rat cortex | Size | 1:500 | |
| 5-HT3A | Primary |
Novus Biologicals NB100-56351 |
RRID:AB_838865 | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:50 | 65 |
| 5-HT7 | Primary |
Novus Biologicals NB100-56352 |
5-hydroxytryptamine receptor 7, RRID:AB_2264237 |
Citations
(6) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 5-Lipoxygenase | Primary |
Novus Biologicals NB110-58748 |
RRID:AB_920580 | Datasheet | Rabbit | Human Lung lysate 0.5 mg/mL | Size | 50 µg/mL | |
| 5-Lipoxygenase | Primary |
Novus Biologicals NB110-58749 |
RRID:AB_920578 | Datasheet | Rabbit | Human Lung lysate 0.5 mg/mL | Size | 10 µg/mL | |
| 53BP1 | Primary |
Abcam ab172580 |
Unmodified, RRID:AB_2924712 |
Citations
(1) Datasheet |
Rabbit | Skin | Size | 1:50 | |
| 53BP1 (phospho S25) | Primary |
Abcam ab70323 |
Phospho,Ser25, RRID:AB_1267592 |
Citations
(1) Datasheet |
Rabbit | Skin | Size | 1:50 | |
| 5T4 | Primary |
R&D Systems AF4975 |
RRID:AB_2207316 | Datasheet | Sheep | Human placenta | Size | 50 µg/mL | 90 |
| 6x His tag | Primary |
Abcam ab252883 |
Epitope tag |
Citations
(1) Datasheet |
Mouse | ClpP1P2 detection for M. smegmatis and M. tuberculosis | Size | 0.02 µg/mL or 0.05 µg/mL | 26–27 |
| 6x His tag | Primary |
Abcam ab206500 |
Epitope tag |
Citations
(1) Datasheet |
Mouse | ClpP1P2 detection for M. smegmatis and M. tuberculosis | Size | 0.02 µg/mL or 0.05 µg/mL | 26–27 |
| 6x-His Tag | Primary |
Novus Biologicals NB100-64768 |
Epitope tag, RRID:AB_963890 | Datasheet | Mouse | rhEDG6 CHO S Tx lysate 0.1 mg/mL, Recombinant EGFP (left) and rhSNAP25-GFP (right) lysate 1.25 mg/mL | Size | 1:250, 1:50 | |
| 6x-His Tag | Primary |
Takara Bio 631212 |
Epitope tag, RRID:AB_2721905 |
Citations
(1) Datasheet |
Mouse | Size | 1:50 | ||
| 6x-His Tag | Primary |
Invitrogen Antibodies 710286 |
Epitope tag, RRID:AB_2532664 |
Citations
(1) Datasheet |
Rabbit | Blood | Size | 55 | |
| 6x-His Tag | Primary |
Invitrogen Antibodies PA1-983B-HRP |
Epitope tag, RRID:AB_2610668 |
Citations
(1) Datasheet |
Rabbit | Blood | Size | 55 | |
| 6x-His tag | Primary |
Invitrogen Antibodies MA1-21315 |
6x-His Tag, RRID:AB_557403 |
Citations
(1) Datasheet |
Mouse | Cell extracts (transduction studies) | 1:2 | ||
| 6x-His Tag | Primary |
Thermo Fisher Scientific MA1-21315-HRP |
Epitope tag, RRID:AB_2536989 | Mouse | Size | 1:50, 1:100 | |||
| 6x-His Tag | Primary |
Abcam ab18184 |
Epitope tag, RRID:AB_444306 |
Citations
(2) Datasheet |
Mouse | Size | |||
| A RAF | Primary |
Abcam ab200653 |
RRID:AB_3086744 | Rabbit | A375 cell line | Size | 1:50 | ||
| A. aeolicus BPL/BioID2 | Primary |
Novus Biologicals NBP2-59940 |
RRID:AB_3349243 | Datasheet | Mouse | Size | 94 | ||
| A1Up | Primary |
Santa Cruz Biotechnology sc-136145 |
RRID:AB_2009286 |
Citations
(1) Datasheet |
Mouse | TE-10, TE-8 | Size | 1:10 | |
| A20 | Primary |
R&D Systems MAB7598 |
RRID:AB_3658849 | Datasheet | Mouse | Jurkat, HepG2, NCI-H460 | Size | 1:20 | 101 |
| A20 | Primary |
Origene TA318419 |
Citations
(1) Datasheet |
Rabbit | Caco cell lysate | Size | |||
| A20 (TNFa-induced protein 1 member 3) | Primary |
Origene TA307167 |
Rabbit | Caco2 cell lysates | Size | 1:50 | |||
| A20/TNFAIP3 | Primary |
Novus Biologicals NBP2-33252 |
RRID:AB_3284683 | Datasheet | Mouse | ||||
| A20/TNFAIP3 | Primary |
Novus Biologicals NBP1-77533 |
RRID:AB_11017330 | Datasheet | Mouse | Jurkat lysate 0.05 mg/mL | Size | 1:100 | 102 |
| A20/TNFAIP3* | Primary |
Cell Signaling Technology 5630 |
RRID:AB_10698880 |
Citations
(2) Datasheet |
Rabbit | A20 | Size | 1:10–1:50 | 97 |
| A29 Antigen | Primary |
BEI Resources NR-627 |
A29 Antigen |
Citations
(1) Datasheet |
Rabbit | Neat supernatants and biotinylated protein standards | 1:100 | ||
| A2BP1 | Primary |
Novus Biologicals NBP2-80553 |
RRID:AB_3409571 | Datasheet | Mouse | 1:20 | |||
| A2BP1 | Primary |
Novus Biologicals NBP2-13169 |
RRID:AB_3260801 | Datasheet | Mouse | Human Brain lysate 0.5 mg/mL | Size | 1:20 | 54 |