Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated and backed by Cell Signaling Technology (CST)
Simple Western Validated Antibodies
Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Citations & Supporting Data | Host Species | Sample | Separation | Dilution | kDa on Simple Western |
---|---|---|---|---|---|---|---|---|---|
α-Actinin-4 | Primary |
GeneTex GTX101669 |
alpha (α) Actinin 4, RRID:AB_10617403 |
Citations
(1) Datasheet |
Rabbit | UC-sEVs and SEC-sEVs | Size | 32 µg/mL | ~100 kDa |
α-Elastin | Primary |
Invitrogen Antibodies PA5-72440 |
Elastin, RRID:AB_2718294 |
Citations
(1) Datasheet |
Rabbit | 1:20 | |||
α-Sarcomeric actin | Primary |
MilliporeSigma A2172 |
Purified rabbit striated muscle actin used as immunogen, RRID:AB_476695 |
Citations
(1) Datasheet |
Mouse | Protein lysates | Size | 1:10,000 | |
α-SMA | Primary |
Abcam ab21027 |
alpha (α) smooth muscle Actin, RRID:AB_1951138 |
Citations
(1) Datasheet |
Goat | Lung fibroblasts | 1:50 | ||
α-SMA | Primary |
Invitrogen Antibodies 14-9760-82 |
alpha (α)-Smooth Muscle Actin, RRID:AB_2572996 |
Citations
(1) Datasheet |
Mouse | Fibroblasts | 1:100 | ||
α-SMA | Primary |
Dako M0851 |
alpha (α) SMA, RRID:AB_2223500 |
Citations
(1) Datasheet |
Mouse | Rabbit corneal fibroblasts treated with TGF beta | Size | 1:100 | 48 |
α-Synuclein | Primary |
Cell Signaling Technology 2642 |
alpha (α)-Synuclein , RRID:AB_2192679 |
Citations
(1) Datasheet |
Rabbit | Size | 1:25 | 21 | |
α-Tubulin | Primary |
Abcam ab52866 |
alpha (α) Tubulin [EP1332Y], RRID:AB_869989 |
Citations
(1) Datasheet |
Rabbit | Cytoplasmic Fraction | Size | 2.7 μg/mL | 54 |
α-tubulin | Primary |
Invitrogen Antibodies PA5-16891 |
alpha (α) Tubulin, RRID:AB_10984790 |
Citations
(1) Datasheet |
Rabbit | 1:1 | |||
α7nAChR | Primary |
Abcam ab216485 |
Citations
(1) Datasheet |
Rabbit | Airway Smooth Muscle (ASM) | 1:50 | |||
β-actin | Primary |
Bioss bs-0061R |
beta (β) Actin, RRID:AB_10855480 |
Citations
(1) Datasheet |
Rabbit | Rat kidney, pancreas, and heart | Size | 1:25, 1:75, 1:100, 1:150, 1:300 | 48 |
β-Actin | Primary |
Proteintech 20536-1-AP |
beta (β) Actin , RRID:AB_10700003 |
Citations
(3) Datasheet |
Rabbit | Protein extract from tissues | 1:200 | ||
β-actin | Primary |
Cell Signaling Technology 5125 |
beta (β) Actin , RRID:AB_1903890 |
Citations
(2) Datasheet |
Rabbit | Frozen samples | 1:2000 | ||
β-Actin | Primary |
Abcam ab213262 |
beta (β) Actin , RRID:AB_2924820 |
Citations
(1) Datasheet |
Rabbit | Liver tissue homogenate (mouse) | |||
β-Actin | Primary |
Wanleibio WL01372 |
beta (β) Actin , RRID:AB_2847841 |
Citations
(1) Datasheet |
Rabbit | Cell lysates | |||
β-actin | Primary |
PTM Bio PTM-5018 |
beta (β) Actin, RRID:AB_3086678 |
Citations
(1) Datasheet |
Mouse | Retinal tissue | Size | 1:50 | |
β-Actin | Primary |
Proteintech 81115-1-RR |
beta (β) Actin, RRID:AB_2923704 |
Citations
(1) Datasheet |
Rabbit | Supernatant from lysate | |||
β-actin | Primary |
ABclonal AC026 |
ACTB, RRID:AB_2768234 |
Citations
(2) Datasheet |
Rabbit | Control tissue samples | |||
β-actin | Primary |
Abcam ab179467 |
actin, RRID:AB_2737344 |
Citations
(1) Datasheet |
Rabbit | Cell lysates | |||
β-Actin | Primary |
ABclonal AC004 |
beta (β) Actin , RRID:AB_2737399 |
Citations
(1) Datasheet |
Mouse | Phf2 KO myoblasts | 1:50 | ||
β-Actin | Primary |
Abcam ab115777 |
beta (β) Actin antibody [SP124], RRID:AB_10899528 |
Citations
(1) Datasheet |
Rabbit | Vascular smooth muscle cells (VSMCs) | 1:150 | ||
β-actin | Primary |
Abcam ab124964 |
alpha (α) smooth muscle Actin [EPR5368], RRID:AB_11129103 |
Citations
(2) Datasheet |
Rabbit | Human Umbilical Vein Endothelial Cells (HUVECs) | 1:400 | ||
β-actin (Mouse monoclonal clone [AC-15]) | Primary |
Abcam ab49900 |
beta (β) Actin , RRID:AB_867494 |
Citations
(1) Datasheet |
Mouse | 1:100 | |||
β-amyloid | Primary |
Santa Cruz Biotechnology sc-28365 |
beta (β) Amyloid, RRID:AB_626669 |
Citations
(2) Datasheet |
Mouse | Fibroblasts, Mouse brain | Size | 1:50 | |
β-catenin | Primary |
ZenBio 201328-5D6 |
Citations
(1) Datasheet |
Mouse | Intestinal tissue | ||||
β-Catenin | Primary |
Cell Signaling Technology 2951 |
Catenin, beta, RRID:AB_1196644 |
Citations
(1) Datasheet |
Total protein extracted (KYSE70 cells) | 1:50 | |||
β-catenin | Primary |
Santa Cruz Biotechnology sc-7963 |
Raised against amino acids 680-781 mapping at the C-terminus of human b-Cat, RRID:AB_626807 |
Citations
(1) Datasheet |
Mouse | Lung cancer cell | Size | 1:50 | |
β-Tubulin | Primary |
MilliporeSigma T8328 |
RRID:AB_1844090 |
Citations
(1) Datasheet |
Mouse | Size | |||
γ-ACTIN | Primary |
GeneTex GTX101794 |
Actin γ 1, RRID:AB_2036153 |
Citations
(1) Datasheet |
Rabbit | Human Stomach Smooth Muscles | Size | 1:100 | 44 |
γ-Tubulin | Primary |
MilliporeSigma T6557 |
Tubulin, gamma, RRID:AB_477584 |
Citations
(2) Datasheet |
Mouse | Cell lysates | 8 µg/mL | ||
1-acyl-sn-glycerol-3-phosphate acyltransferase beta | Primary |
MilliporeSigma HPA019544 |
1-acyl-sn-glycerol-3-phosphate acyltransferase beta, RRID:AB_1844646 |
Citations
(1) Datasheet |
Rabbit Polyclonal | Placenta | Size | 1:100 | |
14-3-3 β | Primary |
Invitrogen Antibodies PA1-37002 |
14-3-3 protein beta (β)/alpha (α), RRID:AB_2217649 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:1000 | |
14-3-3 β/α | Primary |
Cell Signaling Technology 9636 |
Unmodified, RRID:AB_560823 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
14-3-3 ε | Primary |
Cell Signaling Technology 9635 |
Unmodified, RRID:AB_2217758 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
14-3-3 η | Primary |
Cell Signaling Technology 9640 |
Unmodified, RRID:AB_2218073 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
14-3-3 γ | Primary |
Novus Biologicals NB100-406 |
Unmodified, RRID:AB_10002615 | Datasheet | Mouse | HeLa lysate 1.0 mg/mL | Size | 1:12.5 | 36 |
14-3-3 γ | Primary |
Novus Biologicals NBP2-27202 |
Unmodified, RRID:AB_3275422 | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:50 | 33 |
14-3-3 γ | Primary |
Novus Biologicals NBP2-27370 |
Unmodified, RRID:AB_3276333 | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:10 | 34 |
14-3-3 γ | Primary |
Novus Biologicals NBP2-81016 |
RRID:AB_3410086 | Datasheet | Mouse | 1:12.5 | |||
14-3-3 γ | Primary |
Cell Signaling Technology 9637 |
Unmodified, RRID:AB_2218254 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
14-3-3 γ | Primary |
Santa Cruz Biotechnology sc-731 |
Unmodified, RRID:AB_2217962 |
Citations
(1) Datasheet |
Rabbit | Size | 1:100 | 39 | |
14-3-3 τ | Primary |
Cell Signaling Technology 9638 |
Unmodified, RRID:AB_2218251 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
14-3-3 ζ | Primary |
R&D Systems MAB2669 |
Unmodified, RRID:AB_2814945 | Datasheet | Mouse | SH-SY5Y human neuroblastoma cell line | Size | 2 µg/mL | 34 |
14-3-3 ζ/δ | Primary |
Cell Signaling Technology 9639 |
Unmodified, RRID:AB_2273196 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
14-3-3 (pan) | Primary |
Cell Signaling Technology 8312 |
Pan, RRID:AB_10860606 |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
15-PGDH/HPGD | Primary |
R&D Systems AF5660 |
Unmodified, RRID:AB_2120408 | Datasheet | Goat | Human liver tissue | Size | 10 µg/mL | 34 |
15-PGDH/HPGD | Primary |
Novus Biologicals NB200-179 |
Unmodified, RRID:AB_10002546 | Datasheet | Rabbit | Lovo lysate 0.5 mg/mL | Size | 1:500 | 34 |
15-PGDH/HPGD* | Primary |
Cell Signaling Technology 94019 |
Datasheet | Rabbit | RT-4 | Size | 1:50–1:250 | 33 | |
17 β-HSD1/HSD17B1 | Primary |
R&D Systems MAB7178 |
Unmodified, RRID:AB_3658719 | Datasheet | Mouse | Human placenta tissue | Size | 2.5 µg/mL | 40 |
20S Proteasome α3 | Primary |
Santa Cruz Biotechnology sc-166205 |
Unmodified, RRID:AB_2253046 |
Citations
(1) Datasheet |
Mouse | Bone marrow | Size | 1:100 | 33 |
26S proteasome non-ATPase regulatory subunit 8 | Primary |
Santa Cruz Biotechnology sc-514053 |
Citations
(1) Datasheet |
Mouse | HRTPT | Size | 1:50 | Not stated | |
3β-HSD | Primary |
Santa Cruz Biotechnology sc-100466 |
Unmodified, RRID:AB_1118475 |
Citations
(1) Datasheet |
Mouse | Kidneys | Size | 1:50 | 42 |
3β-HSD | Primary |
Santa Cruz Biotechnology sc-28206 |
Unmodified, RRID:AB_2120105 |
Citations
(1) Datasheet |
Rabbit | Testes | Size | 1:100 | 42 |
3-hydroxyanthranilate 3,4-dioxygenase | Primary |
Proteintech 12791-1-AP |
3-hydroxyanthranilate 3,4-dioxygenase, RRID:AB_2115432 |
Citations
(1) Datasheet |
Rabbit | Liver | Size | 1:300 | |
4-1BB/TNFRSF9 | Primary |
R&D Systems AF838 |
Unmodified, RRID:AB_355648 | Datasheet | Goat | HDLM2 human Hodgkin’s lymphoma cells | Size | 1:20 | 58 |
4-1BB/TNFRSF9 | Primary |
R&D Systems MAB8381 |
Unmodified, RRID:AB_3659150 | Datasheet | Mouse | HDLM2 human Hodgkin’s lymphoma cells | Size | 1:20 | 49, 57 |
4-Hydroxynonenal | Primary |
R&D Systems MAB3249 |
RRID:AB_664165 |
Citations
(1) Datasheet |
Mouse | HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) | Size | 5 µg/mL | |
4-Hydroxynonenal (4HNE) | Primary |
Abcam ab46545 |
HNE conjugated to BC., RRID:AB_722490 |
Citations
(1) Datasheet |
Rabbit | Redox EVs | 1:25 | ||
4-Hydroxynonenal (HNE) | Primary |
Cell Biolabs STA-035 |
Citations
(1) Datasheet |
Rabbit | Size | ||||
4E-BP1 | Primary |
R&D Systems AF3227 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_2097980 | Datasheet | Goat | HeLa human cervical epithelial carcinoma cell line | Size | 5 µg/mL | 26 |
4E-BP1 | Primary |
Cell Signaling Technology 4923 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_659944 | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50–1:200 | ||
4E-BP1 | Primary |
Epitomics 1557-1 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_562030 | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50–1:200 | ||
4E-BP1 | Primary |
MilliporeSigma 07-1416 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_1586861 | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50–1:200 | ||
4E-BP1 | Primary |
Cell Signaling Technology 9452 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_331692 |
Citations
(14) Datasheet |
Rabbit | Muscle | Size | 1:50 | |
4E-BP1* | Primary |
Cell Signaling Technology 9644 |
Eukaryotic translation initiation factor 4E-binding protein 1, RRID:AB_2097841 |
Citations
(6) Datasheet |
Rabbit | HepG2 | Size | 1:50 – 1:250 | 24 |
4E-BP2 | Primary |
Cell Signaling Technology 2845 |
Eukaryotic translation initiation factor 4E-binding protein 2, RRID:AB_10699019 |
Citations
(1) Datasheet |
Rabbit | SH-SY5Y | Size, Charge | ||
4F2hc/CD98 (D6O3P) | Primary |
Cell Signaling Technology 13180 |
4F2hc/CD98, RRID:AB_2687475 |
Citations
(1) Datasheet |
Rabbit | HEK293T, MDA-MB-231, PANC-1 | Size | 1:250 | |
4HPPD | Primary |
Proteintech 17004-1-AP |
RRID:AB_2264310 | Rabbit | recombinant protein expression in E. coli | Size | 1:50 | 52 | |
5'-Nucleotidase/CD73 | Primary |
R&D Systems AF5795 |
RRID:AB_1964487 | Datasheet | Sheep | U266 human myeloma cell line | Size | 10 µg/mL | 86 |
5-HT1A | Primary |
Novus Biologicals NBP2-21590 |
RRID:AB_3102030 |
Citations
(5) Datasheet |
Rabbit | ||||
5-HT1D | Primary |
Novus Biologicals NB100-56349 |
RRID:AB_838864 | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:10 | |
5-HT2AR | Primary |
LSBio LS-C172270 |
HTR2A / 5‑HT2A Receptor, RRID:AB_3102031 |
Citations
(2) Datasheet |
Rabbit | Rat cortex | Size | 1:500 | |
5-HT3A | Primary |
Novus Biologicals NB100-56351 |
RRID:AB_838865 | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:50 | 65 |
5-HT7 | Primary |
Novus Biologicals NB100-56352 |
5-hydroxytryptamine receptor 7, RRID:AB_2264237 |
Citations
(6) Datasheet |
Rabbit | Brain | Size | 1:50 | |
5-Lipoxygenase | Primary |
Novus Biologicals NB110-58748 |
RRID:AB_920580 | Datasheet | Rabbit | Human Lung lysate 0.5 mg/mL | Size | 50 µg/mL | |
5-Lipoxygenase | Primary |
Novus Biologicals NB110-58749 |
RRID:AB_920578 | Datasheet | Rabbit | Human Lung lysate 0.5 mg/mL | Size | 10 µg/mL | |
53BP1 | Primary |
Abcam ab172580 |
Unmodified, RRID:AB_2924712 |
Citations
(1) Datasheet |
Rabbit | Skin | Size | 1:50 | |
53BP1 (phospho S25) | Primary |
Abcam ab70323 |
Phospho,Ser25, RRID:AB_1267592 |
Citations
(1) Datasheet |
Rabbit | Skin | Size | 1:50 | |
5T4 | Primary |
R&D Systems AF4975 |
RRID:AB_2207316 | Datasheet | Sheep | Human placenta | Size | 50 µg/mL | 90 |
6x His tag | Primary |
Abcam ab252883 |
Epitope tag |
Citations
(1) Datasheet |
Mouse | ClpP1P2 detection for M. smegmatis and M. tuberculosis | Size | 0.02 µg/mL or 0.05 µg/mL | 26–27 |
6x His tag | Primary |
Abcam ab206500 |
Epitope tag |
Citations
(1) Datasheet |
Mouse | ClpP1P2 detection for M. smegmatis and M. tuberculosis | Size | 0.02 µg/mL or 0.05 µg/mL | 26–27 |
6x-His Tag | Primary |
Novus Biologicals NB100-64768 |
Epitope tag, RRID:AB_963890 | Datasheet | Mouse | rhEDG6 CHO S Tx lysate 0.1 mg/mL, Recombinant EGFP (left) and rhSNAP25-GFP (right) lysate 1.25 mg/mL | Size | 1:250, 1:50 | |
6x-His Tag | Primary |
Takara Bio 631212 |
Epitope tag, RRID:AB_2721905 |
Citations
(1) Datasheet |
Mouse | Size | 1:50 | ||
6x-His Tag | Primary |
Invitrogen Antibodies 710286 |
Epitope tag, RRID:AB_2532664 |
Citations
(1) Datasheet |
Rabbit | Blood | Size | 55 | |
6x-His Tag | Primary |
Invitrogen Antibodies PA1-983B-HRP |
Epitope tag, RRID:AB_2610668 |
Citations
(1) Datasheet |
Rabbit | Blood | Size | 55 | |
6x-His tag | Primary |
Invitrogen Antibodies MA1-21315 |
6x-His Tag, RRID:AB_557403 |
Citations
(1) Datasheet |
Mouse | Cell extracts (transduction studies) | 1:2 | ||
6x-His Tag | Primary |
Thermo Fisher Scientific MA1-21315-HRP |
Epitope tag, RRID:AB_2536989 | Mouse | Size | 1:50, 1:100 | |||
6x-His Tag | Primary |
Abcam ab18184 |
Epitope tag, RRID:AB_444306 |
Citations
(2) Datasheet |
Mouse | Size | |||
A RAF | Primary |
Abcam ab200653 |
RRID:AB_3086744 | Rabbit | A375 cell line | Size | 1:50 | ||
A. aeolicus BPL/BioID2 | Primary |
Novus Biologicals NBP2-59940 |
RRID:AB_3349243 | Datasheet | Mouse | Size | 94 | ||
A1Up | Primary |
Santa Cruz Biotechnology sc-136145 |
RRID:AB_2009286 |
Citations
(1) Datasheet |
Mouse | TE-10, TE-8 | Size | 1:10 | |
A20 | Primary |
R&D Systems MAB7598 |
RRID:AB_3658849 | Datasheet | Mouse | Jurkat, HepG2, NCI-H460 | Size | 1:20 | 101 |
A20 | Primary |
Origene TA318419 |
Citations
(1) Datasheet |
Rabbit | Caco cell lysate | Size | |||
A20 (TNFa-induced protein 1 member 3) | Primary |
Origene TA307167 |
Rabbit | Caco2 cell lysates | Size | 1:50 | |||
A20/TNFAIP3 | Primary |
Novus Biologicals NBP2-33252 |
RRID:AB_3284683 | Datasheet | Mouse | ||||
A20/TNFAIP3 | Primary |
Novus Biologicals NBP1-77533 |
RRID:AB_11017330 | Datasheet | Mouse | Jurkat lysate 0.05 mg/mL | Size | 1:100 | 102 |
A20/TNFAIP3* | Primary |
Cell Signaling Technology 5630 |
RRID:AB_10698880 |
Citations
(2) Datasheet |
Rabbit | A20 | Size | 1:10–1:50 | 97 |
A29 Antigen | Primary |
BEI Resources NR-627 |
A29 Antigen |
Citations
(1) Datasheet |
Rabbit | Neat supernatants and biotinylated protein standards | 1:100 | ||
A2BP1 | Primary |
Novus Biologicals NBP2-80553 |
RRID:AB_3409571 | Datasheet | Mouse | 1:20 | |||
A2BP1 | Primary |
Novus Biologicals NBP2-13169 |
RRID:AB_3260801 | Datasheet | Mouse | Human Brain lysate 0.5 mg/mL | Size | 1:20 | 54 |
A33 | Primary |
R&D Systems MAB30802 |
Datasheet | Mouse | HT-29, COLO 205 | Size | 1:50 | 40 | |
A33 | Primary |
R&D Systems MAB30803 |
RRID:AB_3658065 | Datasheet | Rabbit | Human Colon | Size | 1:50 | 40 |
A33 | Primary |
R&D Systems MAB30804 |
RRID:AB_3658066 | Datasheet | Rabbit | Human Colon | Size | 1:20 | 59 |
AAV B1 | Primary |
Progen Biotechnik 65158 |
Anti-AAV B1 |
Citations
(2) Datasheet |
Mouse | Various rAAV serotypes, including rAAV6 | 2.5 ng/mL | ||
AAV VP1 | Primary |
PROGEN 61056 |
Datasheet | Mouse | AAV purified from HEK293 | Size | 1:10 | 104 | |
AAV VP1/VP2 | Primary |
PROGEN 61057 |
Mouse | AAV2 purified from HEK293 cell lysate | Size | 1:50 | 104, 82 | ||
AAV VP1/VP2/VP3 | Primary |
PROGEN 690058 |
RRID:AB_3068547 | Datasheet | Mouse | Liver cells transduced with AAV2 | Size | 20 µg/mL | |
AAV VP1/VP2/VP3 mouse , B1, lyophilized, purified | Primary |
Progen Biotechnik 61058 |
AAV VP1/VP2/VP3 mouse monoclonal, B1, lyophilized, purified |
Citations
(2) Datasheet |
Mouse | HEK293T | Size | 1:50 | |
AAV VP1/VP2/VP3 [B1] | Primary |
Progen Biotechnik 690058S |
RRID:AB_3068547 | Mouse | AAV | Size | 1 µg/mL | 70, 90, 110 | |
AAV2 Replicase | Primary |
Progen Biotechnik 61069 |
AAV2 Replicase |
Citations
(1) Datasheet |
Mouse | ||||
AAV2 VP1/VP2/VP3 | Primary |
PROGEN 61058-647 |
Datasheet | Mouse | AAV purified from HEK293 | Size, Charge | |||
ABCA1 | Primary |
Novus Biologicals NB400-105 |
RRID:AB_10000630 |
Citations
(1) Datasheet |
Rabbit | Charge | 1:50 | ||
ABCA1 | Primary |
Abcam ab18180 |
Unmodified, RRID:AB_444302 |
Citations
(4) Datasheet |
Mouse | Brain | Size | 1:50 | 270 |
ABCA1 | Primary |
Abcam ab63918 |
HIV1 Nef, RRID:AB_1139525 |
Citations
(2) Datasheet |
Rabbit | Brain tissue and transfected HEK293T cells | 1:40 | ||
ABCB4 | Primary |
LSBio LS-C357461 |
Citations
(1) Datasheet |
Rabbit | HEK293 | ||||
ABCB4 | Primary |
Abcam ab24108 |
Unmodified, RRID:AB_447874 |
Citations
(2) Datasheet |
Mouse | EOMA, MAEC | Size | 1:50 | |
ABCD1/ALD | Primary |
Abcam ab197013 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Skin | Size | 1:500 | |
ABCE1 | Primary |
Abcam ab185548 |
RRID: AB_2858278 |
Citations
(1) Datasheet |
Rabbit | DMEM/RPMI cultured cells | Size | Not specified | |
ABCF2 | Primary |
Novus Biologicals NB400-115 |
RRID:AB_10002669 | Datasheet | Rabbit | HeLa lysate 0.05 mg/mL | Size | 1:1000 | 77 |
ABCG1 | Primary |
Novus Biologicals NB400-132 |
RRID:AB_920491 | Rabbit | Liver lysate 0.1 mg/mL | Size | 1:25 | 60 | |
ABCG1 | Primary |
Abcam ab52617 |
RRID:AB_867471 | Rabbit | Mouse liver lysate | Size | 1:50 | 110-116 | |
ABCG2 | Primary |
Novus Biologicals NBP1-59749 |
RRID:AB_11018021 |
Citations
(1) Datasheet |
Rabbit | HepG2 lysate 0.5 mg/mL | Size | 1:50 | 72 |
ABCG2 | Primary |
Abcam ab24115 |
BCRP/ABCG2 antibody [BXP-53], RRID:AB_447879 |
Citations
(1) Datasheet |
Rat | 3T3, MCF7, H460 | Size | 1:20 | 100 |
ABCG5 | Primary |
Proteintech 27722-1-AP |
RRID:AB_2880952 | Rabbit | Liver lysate 0.1 mg/mL | Size | 10 µg/mL | 66 | |
Abhd5 | Primary |
Novus Biologicals NB110-41576 |
RRID:AB_828505 | Datasheet | Rabbit | MCF-7 lysate 0.5 mg/mL | Size | 1:1000 | 55 |
ABL1 | Primary |
Abcam ab15130 |
c Abl antibody, RRID:AB_301675 |
Citations
(1) Datasheet |
Rabbit | Hypothalamic tissues | 1:100 | ||
Ac-TUBA4 | Primary |
MilliporeSigma T7451 |
RRID: AB_609894 |
Citations
(1) Datasheet |
Mouse | Cell Lysate | Size | 1:100 | 50 |
ACAD8 | Primary |
Epigentek A68138 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Embryo, Muscle | Size | 1:50 | |
ACADL | Primary |
Novus Biologicals NBP1-54936 |
RRID:AB_11037803 | Datasheet | Rabbit | Rat/mouse liver | Size | 1:50 | 48 |
ACADL | Primary |
Aviva Systems Biology arp33855_p050 |
RRID:AB_1293966 | Rabbit | Rat and mouse liver | Size | 1:50 | 47 | |
ACADM/MCAD | Primary |
Abcam ab92461 |
Unmodified, RRID:AB_10563530 |
Citations
(1) Datasheet |
Rabbit | Embryo, Muscle | Size | 1:50 | |
ACADS/SCAD | Primary |
Abcam ab156571 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Embryo, Muscle | Size | 1:100 | |
ACADSB | Primary |
Proteintech 13122-1-AP |
ACADSB, RRID:AB_10638904 |
Citations
(1) Datasheet |
Rabbit | Embryo, Muscle | Size | 1:50 | |
ACADVL/VLCAD | Primary |
Abcam ab155138 |
Unmodified, RRID:AB_2811046 |
Citations
(2) Datasheet |
Rabbit | Embryo, Muscle | Size | 1:50 | |
ACE-2 | Primary |
R&D Systems MAB34372 |
ACE-2, RRID:AB_3658122 |
Citations
(1) Datasheet |
Rabbit | Lungs | Size | ||
ACE/CD143 | Primary |
R&D Systems AF1513 |
RRID:AB_354832 | Datasheet | Goat | Mouse lung tissue | Size | 10 µg/mL | 218 |
ACE2 | Primary |
R&D Systems MAB10821 |
RRID:AB_3657375 | Datasheet | Mouse | Human kidney | Size | 20 µg/mL | |
ACE2 | Primary |
R&D Systems AF3437 |
RRID:AB_2223140 | Datasheet | Goat | Mouse kidney tissue | Size | 2.5 µg/mL | 141 |
ACE2 | Primary |
R&D Systems AF933 |
RRID:AB_355722 |
Citations
(3) Datasheet |
Goat | Human kidney tissue, Recombinant Human ACE-2 Protein, CF (R&D Systems PN 933-ZN-010) | Size | 10 µg/mL, 1:50 | 155, 158 |
ACE2 | Primary |
Abcam ab108252 |
Unmodified, RRID:AB_10864415 |
Citations
(2) Datasheet |
Rabbit | Cornea | Size | 1:100 | |
ACE2 | Primary |
Santa Cruz Biotechnology sc-390581 |
Citations
(1) Datasheet |
Mouse | Size | ||||
ACE2 | Primary |
Abcam ab239924 |
ACE2, RRID:AB_2861381 |
Citations
(1) Datasheet |
Rabbit | Size | 1:50 | ||
ACE2 | Primary |
Abcam ab15348 |
Angiotensin Converting Enzyme 2, RRID:AB_301861 |
Citations
(2) Datasheet |
Rabbit | HAEC | Size | ||
ACE2 | Primary |
Invitrogen Antibodies PA5-20045 |
Angiotensin-converting enzyme 2, RRID:AB_11154258 |
Citations
(1) Datasheet |
Rabbit | HEK293 | Size | 1:35 | 160 |
ACE2 | Primary |
Proteintech 21115-1-AP |
ACE2, RRID:AB_10732845 |
Citations
(1) Datasheet |
Rabbit | Mouse tissues | Size | ||
ACE2 Ectodomain | Primary |
R&D Systems MAB933 |
RRID:AB_2223153 | Mouse | Hamster lung, liver, ileum 0.1 mg/mL | Size | 1:10 | 120 | |
AceCS1* | Primary |
Cell Signaling Technology 3658 |
RRID:AB_2222710 |
Citations
(3) Datasheet |
Rabbit | U-118MG | Size | 1:10–1:50 | 80 |
AceCS2/ACSS1 (E6T5E)* | Primary |
Cell Signaling Technology 37041 |
Datasheet | Rabbit | HeLa | Size | 1:10–1:50 | 70 | |
Acetyl-α-Tubulin (Lys40) (D20G3) | Primary |
Cell Signaling Technology 5335 |
Acetyl-Lys40, RRID:AB_10544694 |
Citations
(3) Datasheet |
Rabbit | Brain | Size | 1:50 | |
Acetyl-CoA Carboxylase | Primary |
R&D Systems AF6898 |
RRID:AB_10920935 | Datasheet | Sheep | A431 human epithelial carcinoma cells and C2C12 mouse myoblast cells | Size | 1:20 | 380–400 |
Acetyl-CoA Carboxylase | Primary |
Cell Signaling Technology 3662 |
Unmodified, RRID:AB_2219400 |
Citations
(6) Datasheet |
Rabbit | Muscle, LN-229, NCH644, U87/WT | Size | 1:20, 1:25 | |
Acetyl-CoA Carboxylase (C83B10)* | Primary |
Cell Signaling Technology 3676 |
RRID:AB_2219397 |
Citations
(6) Datasheet |
Rabbit | A431 + EGF | Size | 1:10–1:50 | 220 |
Acetyl-CoA Carboxylase 1 | Primary |
Cell Signaling Technology 4190 |
Unmodified, RRID:AB_10547752 |
Citations
(4) Datasheet |
Rabbit | Liver | Size | ||
Acetyl-Histone H2B (Lys12) | Primary |
Cell Signaling Technology 5410 |
Acetyl Lys12, RRID:AB_10694548 |
Citations
(1) Datasheet |
Rabbit | Stem cell | Size | 1:100 | |
Acetyl-Histone H3 (Lys14) (D4B9)* | Primary |
Cell Signaling Technology 7627 |
Acetyl Lys14, RRID:AB_10839410 | Datasheet | Rabbit | 293T+ ACK1-Myc-Tag | Size | 1:10–1:50 | 24 |
Acetyl-Histone H3 (Lys27) | Primary |
Cell Signaling Technology 4353 |
Acetyl-Lys27, RRID:AB_10545273 | Datasheet | Rabbit | Histone extraction of OCI-LY3 cells untreated and treated with 10nM Panobinostat | Size | 1:50, 1:150, 1:450 | 25 |
Acetyl-Histone H3 (Lys9) (C5B11) | Primary |
Cell Signaling Technology 9649 |
Unmodified, RRID:AB_823528 |
Citations
(1) Datasheet |
Rabbit | Muscle | Size | ||
Acetyl-Histone H4 | Primary |
MilliporeSigma 06-598 |
RRID:AB_2295074 | Datasheet | Rabbit | Histone extraction of OCI-LY3 cells untreated and treated with 10nM Panobinostat | Size | 1:50, 1:150, 1:450 | 23 |
Acetyl-Histone H4 (Lys16) (E2B8W)* | Primary |
Cell Signaling Technology 13534 |
Acetyl-Lys16, RRID:AB_2687581 | Datasheet | Rabbit | Jurkat | Size | 1:10–1:50 | 24 |
Acetylated α Tubulin | Primary |
Santa Cruz Biotechnology sc-23950 |
Unmodified, RRID:AB_628409 |
Citations
(1) Datasheet |
Mouse | Brain | Size | ||
Acetylated a-Tubulin | Primary |
MilliporeSigma T6793 |
Acetylated, RRID:AB_477585 |
Citations
(2) Datasheet |
Mouse | Mouse brain lysate | Size | 1:30,000, 1:300,000, 1:1,500,000 | 53 |
Acetylated FOXO1 | Primary |
Invitrogen Antibodies PA5-104560 |
Acetyl-FOXO1 (Lys294), RRID:AB_2816035 |
Citations
(1) Datasheet |
Rabbit | Cell lysates | |||
Acetylated-Lysine | Primary |
Cell Signaling Technology 9441 |
Acetyl, RRID:AB_331805 |
Citations
(3) Datasheet |
Rabbit | Liver, Spinal cord | Size, Charge | 1:65, 1:100 | 7.3, 5.6 |
Acetylated-Lysine | Primary |
Cell Signaling Technology 9681 |
RRID:AB_331799 | Datasheet | Mouse | Mouse heart | Size | 1:20, 1:50, 1:200 | |
AChE | Primary |
Abcam ab31276 |
Human Acetylcholinesterase, RRID:AB_722529 |
Citations
(1) Datasheet |
Goat | NE-4C cell lysate | 1:50 | ||
ACLY | Primary |
MilliporeSigma SAB4501964 |
RRID:AB_10746187 | Rabbit | Mouse liver 0.1 mg/mL | Size | 1:50 | 116 | |
Aconitase 1 | Primary |
Novus Biologicals NBP1-19412 |
Unmodified, RRID:AB_1642435 |
Citations
(1) Datasheet |
Rabbit | Adipose | Size | 100 | |
Aconitase 2 | Primary |
Abcam ab129069 |
Aconitate hydratase, mitochondrial, RRID:AB_11144142 |
Citations
(1) Datasheet |
Rabbit | Brain, Diaphragm, Heart, Kidneys, Muscle | Size | 1:200 | |
Acrp30 | Primary (Biotin) |
R&D Systems BAF1065 |
RRID:AB_355816 | Datasheet | Goat | Human Serum | Size | 1:50 | 40 |
ACSF2 | Primary |
Novus Biologicals NBP2-47558 |
RRID:AB_3311201 | Datasheet | Rabbit | Size | |||
ACSL1 | Primary |
Novus Biologicals NB110-99585 |
RRID:AB_1502544 | Datasheet | Rabbit | HepG2 lysate 0.5 mg/mL | Size | 1:100 | 65 |
ACSL3 | Primary |
Novus Biologicals NBP2-15252 |
RRID:AB_3262717 |
Citations
(1) Datasheet |
Rabbit | Mouse brain (hippocampus) tissue | Size | ||
ACSL4 | Primary |
Santa Cruz Biotechnology sc-365230 |
RRID:AB_10843105 | Mouse | Rat and Mouse Liver | Size | 1:50 | 66 | |
ACSL4* | Primary |
Cell Signaling Technology 38493 |
Datasheet | Rabbit | Huh-7 | Size | 1:10–1:50 | 80 | |
ACSL5 | Primary |
Novus Biologicals NBP1-59645 |
RRID:AB_11031095 | Datasheet | Rabbit | Mouse liver lysate | Size | ||
Actin | Primary |
Novus Biologicals NB100-74340 |
RRID:AB_1048019 | Datasheet | Mouse | HeLa lysate 0.1 mg/mL | Size | 1:25 | 50 |
Actin | Primary |
Novus Biologicals NBP2-80561 |
RRID:AB_3409606 | Datasheet | Mouse | 1:25 | |||
Actin | Primary |
MilliporeSigma MAB1501 |
Pan, RRID:AB_2223041 |
Citations
(5) Datasheet |
Mouse | Spinal cord | Size | 1:100 | 49 |
Actin | Primary |
MilliporeSigma A4700 |
RRID:AB_476730 | Mouse | HEK293, Mouse Brain, C. elegans | Size | 1:200 | 49 | |
Actin | Primary |
MilliporeSigma A2066 |
Unmodified, RRID:AB_476693 |
Citations
(3) Datasheet |
Rabbit | Heart | Size | 1:20 | |
Actin | Primary |
MilliporeSigma A3853 |
Pan Actin, RRID:AB_262137 |
Citations
(3) Datasheet |
Mouse | UMNSAH/DF-1, Embryo | Size | 1:25 | |
Actin | Primary |
Santa Cruz Biotechnology sc-1616 |
Unmodified, RRID:AB_630836 |
Citations
(3) Datasheet |
Goat | Vero-B4, Liver | Size | ||
Actin | Primary |
MilliporeSigma MAB1501R |
Citations
(2) Datasheet |
Mouse | RAW 264.7 | Size | 1:1000 | ||
Actin γ 1 | Primary |
Novus Biologicals NB600-533 |
RRID:AB_10003448 | Datasheet | Rabbit | HeLa lysate 1.0 mg/mL | Size | 1:100 | 49 |
Actin (C-2) | Primary |
Santa Cruz Biotechnology sc-8432 |
ACTG1, ACTC1, ACTA1, ACTA2, ACTG2, ACTB, RRID:AB_626630 |
Citations
(1) Datasheet |
Mouse | Mouse brain lysate | Size | 1:50 | |
Actin (Muscle Specific) | Primary |
Novus Biologicals NBP2-34634 |
RRID:AB_3290061 | Datasheet | Mouse | ||||
Actin, Cardiac | Primary |
MilliporeSigma A9357 |
Unmodified, RRID:AB_1840685 |
Citations
(1) Datasheet |
Mouse | Heart | Size | ||
Actin, cytoplasmic 1 | Primary |
Invitrogen Antibodies MA5-15452 |
beta (β) Actin, RRID:AB_11001306 |
Citations
(1) Datasheet |
Mouse Monoclonal | A-549,HCC1806,HCC1937,hTERT-HME1 | Size | 1:100 | |
Activated H-Ras | Primary |
MilloporeSigma OP38 |
Cpd, RRID:AB_2083903 |
Citations
(1) Datasheet |
Mouse | Cell lysates | 1:50 | ||
Active caspase-3 | Primary |
BD Biosciences 560626 |
Caspase-3 Active Form, RRID:AB_1727414 |
Citations
(1) Datasheet |
Rabbit | ||||
Active Caspase3 | Primary |
Abcam ab32042 |
Cleaved Caspase-3, RRID:AB_725947 |
Citations
(1) Datasheet |
Rabbit | K562 cells -/+ Imatinib | Charge | 1:50 | |
Active YAP1 | Primary |
Abcam ab205270 |
Transcriptional coactivator YAP1, RRID:AB_2813833 |
Citations
(1) Datasheet |
Rabbit | Heart | Size | 1:200 | |
Activin Receptor Type IIA/ACVR2A | Primary |
Abcam ab134082 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Blood | Size | 78 | |
Activin Receptor Type IIB/ACVR2B | Primary |
Abcam ab128544 |
Unmodified, RRID:AB_11142845 |
Citations
(1) Datasheet |
Rabbit | Blood | Size | 84 | |
Activin Receptor Type-2B (ACVR2B, ACTR-IIB) (Biotin) | Primary |
US Biological A0856-10B |
Unmodified |
Citations
(1) Datasheet |
Goat | Blood | Size | 78 | |
ACTR-IIA | Primary |
Santa Cruz Biotechnology sc-515826 |
Unmodified |
Citations
(1) Datasheet |
Mouse | Blood | Size | 78 | |
ACTR-IIB | Primary |
Santa Cruz Biotechnology sc-376593 |
Unmodified, RRID:AB_11150660 |
Citations
(1) Datasheet |
Mouse | Blood | Size | 84 | |
ACVR2A (aa 24-133) | Primary |
Creative Diagnostics DPAB-DC3752 |
Unmodified, RRID:AB_2500994 |
Citations
(1) Datasheet |
Mouse | Blood | Size | 78 | |
Adalimumab (Anti-Idiotype) | Primary |
R&D Systems MAB9616 |
RRID:AB_3659486 | Datasheet | Rabbit | Purified Adalimumab spiked in human serum | Size, Charge | 1:1000 | |
ADAM10 | Primary |
Cell Signaling Technology 14194 |
ADAM10, RRID:AB_2798420 |
Citations
(1) Datasheet |
Rabbit | Size | 60, 100 |