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Simple Western Antibody Database

Find Antibodies Validated for Simple Western

Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.

How do antibodies become validated for Simple Western?

  • R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
  • Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
  • Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.

You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.

*Antibodies validated and backed by Cell Signaling Technology (CST)

Simple Western Validated Antibodies


Filter Antibodies: (6294)
Protein Target Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species Sample Separation Dilution kDa on Simple Western
α-Actinin-4 Primary GeneTex
GTX101669
alpha (α) Actinin 4 Citations (1)
Datasheet
Rabbit UC-sEVs and SEC-sEVs Size 32 µg/ml ~100 kDa
α-Elastin Primary Invitrogen Antibodies
PA5-72440
Elastin Citations (1)
Datasheet
Rabbit 1:20
α-Sarcomeric actin Primary MilliporeSigma
A2172
Purified rabbit striated muscle actin used as immunogen Citations (1)
Datasheet
Mouse Protein lysates Size 1:10,000
α-SMA Primary Abcam
ab21027
alpha (α) smooth muscle Actin Citations (1)
Datasheet
Goat Lung fibroblasts 1:50
α-SMA Primary Invitrogen Antibodies
14-9760-82
alpha (α)-Smooth Muscle Actin Citations (1)
Datasheet
Mouse Fibroblasts 1:100 Not specified
α-Synuclein Primary Cell Signaling Technology
2642
alpha (α)-Synuclein Citations (1)
Datasheet
Rabbit Size 1:25 21
α-Tubulin Primary Abcam
ab52866
alpha (α) Tubulin [EP1332Y] Citations (1)
Datasheet
Rabbit Cytoplasmic Fraction Size 2.7 (μg/ml) 54
α7nAChR Primary Abcam
ab216485
Citations (1)
Datasheet
Rabbit Airway Smooth Muscle (ASM) 1:50 N/A
β-actin Primary PTM Bio
PTM-5018
beta (β) Actin Citations (1)
Datasheet
Mouse Retinal tissue Size 1:50
β-Actin Primary Proteintech
81115-1-RR
beta (β) Actin Citations (1)
Datasheet
Rabbit Supernatant from lysate
β-actin Primary ABclonal
AC026
ACTB Citations (1)
Datasheet
Rabbit Control tissue samples
β-actin Primary Abcam
ab179467
actin Citations (1)
Datasheet
Rabbit Cell lysates
β-Actin Primary ABclonal
AC004
beta (β) Actin Citations (1)
Datasheet
Mouse Phf2 KO myoblasts 1:50 Not specified
β-actin Primary Abcam
ab124964
alpha (α) smooth muscle Actin [EPR5368] Citations (1)
Datasheet
Rabbit Human Umbilical Vein Endothelial Cells (HUVECs) 1:400 Not specified
β-Actin Primary Proteintech
20536-1-AP
beta (β) Actin Citations (2)
Datasheet
Rabbit Protein extract from tissues 1:200 Not Specified
β-actin Primary Cell Signaling Technology
5125
beta (β) Actin Citations (1)
Datasheet
Rabbit Frozen samples 1:2000 Not specified
β-Actin Primary Abcam
ab213262
beta (β) Actin Citations (1)
Datasheet
Rabbit Liver tissue homogenate (mouse) Not specified Not specified
β-Actin Primary Wanleibio
WL01372
beta (β) Actin Citations (1)
Datasheet
Rabbit Cell lysates Not Specified Not Specified
β-actin (Mouse monoclonal clone [AC-15]) Primary Abcam
ab49900
beta (β) Actin Citations (1)
Datasheet
Mouse 1:100
β-amyloid Primary Santa Cruz Biotechnology
sc-28365
beta (β) Amyloid Citations (2)
Datasheet
Mouse Fibroblasts, Mouse brain Size 1:50
β-catenin Primary ZenBio
201328-5D6
Citations (1)
Datasheet
Mouse Intestinal tissue Not specified Not specified
β-Catenin Primary Cell Signaling Technology
2951
Catenin, beta Citations (1)
Datasheet
Total protein extracted (KYSE70 cells) 1:50 Not explicitly mentioned
β-catenin Primary Santa Cruz Biotechnology
sc-7963
Raised against amino acids 680-781 mapping at the C-terminus of human b-Cat Citations (1)
Datasheet
Mouse Lung cancer cell Size 1:50
β-Tubulin Primary MilliporeSigma
T8328
Citations (1)
Datasheet
Mouse Size
γ-ACTIN Primary GeneTex
GTX101794
Actin γ 1 Citations (1)
Datasheet
Rabbit Human Stomach Smooth Muscles Size 1:100 44
γ-Tubulin Primary MilliporeSigma
T6557
Tubulin, gamma Citations (1)
Datasheet
Mouse Cell lysates 8 µg/mL Not specified
1-acyl-sn-glycerol-3-phosphate acyltransferase beta Primary MilliporeSigma
HPA019544
1-acyl-sn-glycerol-3-phosphate acyltransferase beta Citations (1)
Datasheet
Rabbit Polyclonal Placenta Size 1:100
14-3-3 β Primary Invitrogen Antibodies
PA1-37002
14-3-3 protein beta (β)/alpha (α) Citations (1)
Datasheet
Rabbit Brain Size 1:1000
14-3-3 β/α Primary Cell Signaling Technology
9636
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 ε Primary Cell Signaling Technology
9635
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 η Primary Cell Signaling Technology
9640
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 γ Primary Novus Biologicals
NB100-406
Unmodified Datasheet Mouse HeLa lysate 1.0 mg/mL Size 1:12.5 36
14-3-3 γ Primary Novus Biologicals
NBP2-27202
Unmodified Datasheet Rabbit Human Brain lysate 0.5 mg/mL Size 1:50 33
14-3-3 γ Primary Novus Biologicals
NBP2-27370
Unmodified Datasheet Rabbit Human Brain lysate 0.5 mg/mL Size 1:10 34
14-3-3 γ Primary Novus Biologicals
NBP2-81016
Datasheet Mouse 1:12.5
14-3-3 γ Primary Cell Signaling Technology
9637
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 γ Primary Santa Cruz Biotechnology
sc-731
Unmodified Citations (1)
Datasheet
Rabbit Size 1:100 39
14-3-3 τ Primary Cell Signaling Technology
9638
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 ζ Primary R&D Systems
MAB2669
Unmodified Datasheet Mouse SH-SY5Y human neuroblastoma cell line Size 2 µg/mL 34
14-3-3 ζ/δ Primary Cell Signaling Technology
9639
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 (pan) Primary Cell Signaling Technology
8312
Pan Citations (1)
Datasheet
Rabbit Brain Size 1:50
15-PGDH/HPGD Primary R&D Systems
AF5660
Unmodified Datasheet Goat Human liver tissue Size 10 µg/mL 34
15-PGDH/HPGD Primary Novus Biologicals
NB200-179
Unmodified Datasheet Rabbit Lovo lysate 0.5 mg/mL Size 1:500 34
15-PGDH/HPGD* Primary Cell Signaling Technology
94019
Datasheet Rabbit RT-4 Size 1:50–1:250 33
17 β-HSD1/HSD17B1 Primary R&D Systems
MAB7178
Unmodified Datasheet Mouse Human placenta tissue Size 2.5 µg/mL 40
20S Proteasome α3 Primary Santa Cruz Biotechnology
sc-166205
Unmodified Citations (1)
Datasheet
Mouse Bone marrow Size 1:100 33
3β-HSD Primary Santa Cruz Biotechnology
sc-100466
Unmodified Citations (1)
Datasheet
Mouse Kidneys Size 1:50 42
3β-HSD Primary Santa Cruz Biotechnology
sc-28206
Unmodified Citations (1)
Datasheet
Rabbit Testes Size 1:100 42
3-hydroxyanthranilate 3,4-dioxygenase Primary Proteintech
12791-1-AP
3-hydroxyanthranilate 3,4-dioxygenase Citations (1)
Datasheet
Rabbit Liver Size 1:300
4-1BB/TNFRSF9 Primary R&D Systems
AF838
Unmodified Datasheet Goat HDLM2 human Hodgkin’s lymphoma cells Size 1:20 58

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