Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated and backed by Cell Signaling Technology (CST)
Simple Western Validated Antibodies
Filter Antibodies: (6443)
| Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Citations & Supporting Data | Host Species | Sample | Separation | Dilution | kDa on Simple Western |
|---|---|---|---|---|---|---|---|---|---|
| HIF-1 α | Primary |
Novus Biologicals NBP2-75978 |
HIF-1 α (2443C) | Datasheet | Rabbit | HepG2 +/- DFO cell lysate | Size | 20 µg/mL | 116 |