Lightning-Link (R) PerCP: Conjugation Kits

Easy PerCP labeling of your antibody with 30 seconds hands-on time. PerCP is a small 35kDa phycobiliprotein isolated from red algae. It has a strong absorption peak at 482nm and a secondary absorption peak at 442nm. PerCP has a large stokes shift (195nm) with its maximum emission peak at 677nm. PerCP The protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting.

The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.

Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates.

As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.