5-HT2A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26091

Novus Biologicals, part of Bio-Techne
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NBP2-26091
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all 5-HT2A Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QRSIHREPGSYTGR corresponding to internal region according to NP_000612.1, NP_001159419.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_000612.1; NP_001159419.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 5-HT2A Antibody

Immunocytochemistry/ Immunofluorescence: 5-HT2A Antibody [NBP2-26091]

Immunocytochemistry/ Immunofluorescence: 5-HT2A Antibody [NBP2-26091]

Immunocytochemistry/Immunofluorescence: 5-HT2A Antibody [NBP2-26091] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: 5-HT2A Antibody [NBP2-26091]

Immunohistochemistry-Paraffin: 5-HT2A Antibody [NBP2-26091]

Immunohistochemistry-Paraffin: 5-HT2A Antibody [NBP2-26091] - Staining of paraffin embedded Human Cerebellum. Antibody at 2 ug/mL. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: 5-HT2A Antibody [NBP2-26091]

Flow Cytometry: 5-HT2A Antibody [NBP2-26091]

Flow Cytometry: 5-HT2A Antibody [NBP2-26091] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for 5-HT2A Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

2 ug/mL

Peptide ELISA

Detection limit 1:128000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 5-HT2A

The 5HT2A Receptor gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene areassociated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated withresponse to the antidepressant citalopram in patients with major depressive disorder (MDD). MDD patients who also havea mutation in intron 2 of this gene show a significantly reduced response to citalopram as this antidepressantdownregulates expression of this gene. Multiple transcript variants encoding different isoforms have been found forthis gene. (provided by RefSeq)

Long Name

5-Hydroxytryptamine Receptor 2A

Alternate Names

5HT2A, HTR2A

Entrez Gene IDs

3356 (Human)

Gene Symbol

HTR2A

Additional 5-HT2A Products

Product Documents for 5-HT2A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 5-HT2A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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