Actin Gamma 1 Antibody (2A3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97720

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Chicken, Rabbit, Zebrafish

Cited:

Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2A3

Format

BSA Free

Concentration

1 mg/ml

View all Actin Gamma 1 Antibodies »

Product Specifications

Immunogen

Derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of gamma-cytoplasmic actin with an acetylated N-terminus (Ac-EEEIAALVI-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue. The antibody does not cross react with other actin isoforms.

Reactivity Notes

The epitope recognized is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This mouse monoclonal IgG2b antibody highly specific for gamma-cytoplasmic actin (Actin Gamma 1; encoded by ACTG1). It does not cross-react with the Actin Gamma 1 isoform expressed in the enteric smooth muscle tissues (actin gamma 2; encoded by ACTG2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Scientific Data Images for Actin Gamma 1 Antibody (2A3) - BSA Free

Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody (2A3) - BSA Free [NBP1-97720]

Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody (2A3) - BSA Free [NBP1-97720]

Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody (2A3) [NBP1-97720] - Methanol fixed human dermal fibroblasts immunostained with 2A3 (1:500)
Immunohistochemistry: Actin Gamma 1 Antibody (2A3) - BSA Free [NBP1-97720]

Immunohistochemistry: Actin Gamma 1 Antibody (2A3) - BSA Free [NBP1-97720]

Immunohistochemistry: Actin Gamma 1 Antibody (2A3) [NBP1-97720] - Reactivity of NBP1-97720 (up to a 1:1000 dilution) in the epithelial villi and smooth muscle cells in swine colon.

Applications for Actin Gamma 1 Antibody (2A3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:100 - 1:500

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:1000-1:5000
Application Notes
Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:500 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting applications.

This antibody is CyTOF ready..

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, pH 7.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Actin Gamma 1

Actin, gamma 1 (ACTG1) is a member of the actin protein family, a highly conserved protein group with three main isoforms: alpha, beta and gamma. Alpha actins are mainly expressed in muscle tissues where they are a major constituent of the contractile apparatus, whereas beta and gamma actin proteins are widely expressed in most cell types where they are predominantly involved in cell motility and cytoskeleton maintenance. Actin gamma 1 is a cytoplasmic actin isoform usually found in nonmuscle cells.

Actin Gamma 1 antibodies serve as excellent loading controls, due to the proteins' near ubiquitous expressn and strong conservation across most species. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Actin Gamma 1 antibody serves as an excellent loading control.

Alternate Names

ACT, ACTB, ACTG, actin, cytoplasmic 2, actin, gamma 1, cytoskeletal gamma-actin, deafness, autosomal dominant 20; deafness, autosomal dominant 26, DFNA20, DFNA26, Gamma-actin

Entrez Gene IDs

71 (Human); 11465 (Mouse); 287876 (Rat)

Gene Symbol

ACTG1

UniProt

P63261 (Human)

Additional Actin Gamma 1 Products

Product Documents for Actin Gamma 1 Antibody (2A3) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Actin Gamma 1 Antibody (2A3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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