Actin Gamma 1 Antibody (2A3) [Janelia Fluor® 525]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97720JF525
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Chicken, Rabbit, Zebrafish
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Janelia Fluor 525
Antibody Source
Monoclonal Mouse IgG2B Clone # 2A3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of gamma-cytoplasmic actin with an acetylated N-terminus (Ac-EEEIAALVI-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue. The antibody does not cross react with other actin isoforms.
Reactivity Notes
The epitope recognized is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This mouse monoclonal IgG2b antibody highly specific for gamma-cytoplasmic actin (Actin Gamma 1; encoded by ACTG1). It does not cross-react with the Actin Gamma 1 isoform expressed in the enteric smooth muscle tissues (actin gamma 2; encoded by ACTG2).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Actin Gamma 1 Antibody (2A3) [Janelia Fluor® 525]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined..
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Actin Gamma 1
Actin Gamma 1 antibodies serve as excellent loading controls, due to the proteins' near ubiquitous expressn and strong conservation across most species. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Actin Gamma 1 antibody serves as an excellent loading control.
Alternate Names
ACT, ACTB, ACTG, actin, cytoplasmic 2, actin, gamma 1, cytoskeletal gamma-actin, deafness, autosomal dominant 20; deafness, autosomal dominant 26, DFNA20, DFNA26, Gamma-actin
Gene Symbol
ACTG1
Additional Actin Gamma 1 Products
Product Documents for Actin Gamma 1 Antibody (2A3) [Janelia Fluor® 525]
Product Specific Notices for Actin Gamma 1 Antibody (2A3) [Janelia Fluor® 525]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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