Adenosine A3 R Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NLS689
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Porcine, Canine, Equine, Monkey, Primate
Cited:
Human, Mouse
Predicted:
Opossum (100%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human Adenosine A3 R.
Epitope
3rd cytoplasmic domain
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 30641086). Predicted cross-reactivity based on sequence identity: Marmoset (100%).
Specificity
Human ADORA3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Product can be stored undiluted at 4C for up to 1 month.
Scientific Data Images for Adenosine A3 R Antibody
Western Blot: Adenosine A3 R Antibody [NLS689]
Western Blot: Adenosine A3 R Antibody [NLS689] - EGFR and adenosine signaling pathways are involved in CD73-dependent EMT in HNSCC cells (A) CAL27 cells were treated with siRNA targeting CD73 or control siRNA followed by incubation with MRS3588 for another 36 h. E-cadherin, N-cadherin, Vimentin and Slug levels were evaluated. GAPDH was the internal standard for protein loading. One-way ANOVA with post-Dunnett analysis was performed using GraphPad Prism5.*P < 0.05, versus the si-control group, #P < 0.05, versus the CD73-si group. (n = 3). Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/article/11435/text/), licensed under a CC-BY license.Immunohistochemistry-Paraffin: Adenosine A3 R Antibody [NLS689]
Immunohistochemistry-Paraffin: Adenosine A3 R Antibody [NLS689] - Analysis of anti-Adenosine A3 Receptor / ADORA3 antibody with human tonsil, squamous epithelium at dilution 20-30 ug/ml.Immunohistochemistry: Adenosine A3 R Antibody [NLS689] -
Immunohistochemistry: Adenosine A3 R Antibody [NLS689] - si-CD73 inhibits HNSCC tumor growth & suppress EMT in vivo(A) Tumor growth curve of siRNA-CD73 mice & control mice. Data represent the mean ± SEM. of eight mice in each group. *P < 0.05, **P < 0.01, ***P < 0.001 by the Student's t-test; (B) Dissected tumors were photographed. The tumor volume & weight were measured. ***P < 0.001 by the Student's t-test; (C) Representative images of immunohistochemical analysis of CD73, p-EGFR, p-Src, A3R, Slug, E-cad, N-cad & Vimentin in tumors, (Scale bars = 100 μm). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.11435), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Adenosine A3 R Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
20-30 ug/ml
Application Notes
Use in Flow reported in scientific literature (PMID:34536555). Use in IHC and WB reported in scientific literature (PMID: 27557512). . Use in ICC/IF reported in scientific literature (PMID: 30641086). ..
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Background: Adenosine A3 R
Long Name
Adenosine A3 Receptor
Alternate Names
A3AR, AD026, Adenosine A3R, ADORA3
Entrez Gene IDs
140 (Human)
Gene Symbol
ADORA3
UniProt
Additional Adenosine A3 R Products
Product Documents for Adenosine A3 R Antibody
Product Specific Notices for Adenosine A3 R Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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