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Adenosine A3 R Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NLS689

Novus Biologicals, part of Bio-Techne
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NLS689

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Porcine, Canine, Equine, Monkey, Primate

Cited:

Human, Mouse

Predicted:

Opossum (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human Adenosine A3 R.

Epitope

3rd cytoplasmic domain

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30641086). Predicted cross-reactivity based on sequence identity: Marmoset (100%).

Specificity

Human ADORA3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for Adenosine A3 R Antibody

Western Blot: Adenosine A3 R Antibody [NLS689]

Western Blot: Adenosine A3 R Antibody [NLS689]

Western Blot: Adenosine A3 R Antibody [NLS689] - EGFR and adenosine signaling pathways are involved in CD73-dependent EMT in HNSCC cells (A) CAL27 cells were treated with siRNA targeting CD73 or control siRNA followed by incubation with MRS3588 for another 36 h. E-cadherin, N-cadherin, Vimentin and Slug levels were evaluated. GAPDH was the internal standard for protein loading. One-way ANOVA with post-Dunnett analysis was performed using GraphPad Prism5.*P < 0.05, versus the si-control group, #P < 0.05, versus the CD73-si group. (n = 3). Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/article/11435/text/), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Adenosine A3 R Antibody [NLS689]

Immunohistochemistry-Paraffin: Adenosine A3 R Antibody [NLS689]

Immunohistochemistry-Paraffin: Adenosine A3 R Antibody [NLS689] - Analysis of anti-Adenosine A3 Receptor / ADORA3 antibody with human tonsil, squamous epithelium at dilution 20-30 ug/ml.
Adenosine A3 R Antibody

Immunohistochemistry: Adenosine A3 R Antibody [NLS689] -

Immunohistochemistry: Adenosine A3 R Antibody [NLS689] - si-CD73 inhibits HNSCC tumor growth & suppress EMT in vivo(A) Tumor growth curve of siRNA-CD73 mice & control mice. Data represent the mean ± SEM. of eight mice in each group. *P < 0.05, **P < 0.01, ***P < 0.001 by the Student's t-test; (B) Dissected tumors were photographed. The tumor volume & weight were measured. ***P < 0.001 by the Student's t-test; (C) Representative images of immunohistochemical analysis of CD73, p-EGFR, p-Src, A3R, Slug, E-cad, N-cad & Vimentin in tumors, (Scale bars = 100 μm). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.11435), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Adenosine A3 R Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

20-30 ug/ml
Application Notes
Use in Flow reported in scientific literature (PMID:34536555). Use in IHC and WB reported in scientific literature (PMID: 27557512). . Use in ICC/IF reported in scientific literature (PMID: 30641086). ..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: Adenosine A3 R

A3 Adenosine Receptor is a widely expressed receptor that mediates the multiple functions of adenosine. For example, this receptor is believed to mediate the protective effect of adenosine released during cardiac ischemia. In lungs, the receptor mediates inhibition of eosinophil chemotaxis and may be useful in the treatment of eosinophil-dependent diseases such as asthma and rhinitis. Adenosine A3 Receptor has been reported mostly in brain, lung, liver, heart, kidney, and testis. ESTs have been isolated from heart/melanocyte/uterus, kidney, pituitary, and placenta libraries.

Long Name

Adenosine A3 Receptor

Alternate Names

A3AR, AD026, Adenosine A3R, ADORA3

Entrez Gene IDs

140 (Human)

Gene Symbol

ADORA3

UniProt

Additional Adenosine A3 R Products

Product Documents for Adenosine A3 R Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Adenosine A3 R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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