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AHR Antibody [Alexa Fluor® 700]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2289AF700

Novus Biologicals, part of Bio-Techne
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NB100-2289AF700

Key Product Details

Species Reactivity

Human, Mouse, Rat, Guinea Pig

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Simple Western, Western Blot

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Bacterially expressed human Aryl hydrocarbon Receptor (C-terminus). [UniProt# P35869]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23887904). Porcine reactivity reported from a verified customer review.

Localization

First cytoplasmic, upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AHR Antibody [Alexa Fluor® 700]

AHR Antibody [Alexa Fluor® 700]

AHR Antibody [Alexa Fluor® 700] [NB100-2289AF700] -

AHR Antibody [Alexa Fluor® 700] [NB100-2289AF700] - Vial of Alexa Fluor 700 conjugated antibody. Alexa Fluor 700 is optimally excited at 696 nm by the Red laser (633 or 640 nm) and has an emission maximum of 720 nm.
AHR Antibody [Alexa Fluor® 700]

Flow Cytometry: AHR Antibody [Alexa Fluor® 700] [NB100-2289AF700] -

Flow Cytometry: AHR Antibody [Alexa Fluor® 700] [NB100-2289AF700] - Presence & frequency of ILC subtypes within the meninges of severe TBI patients.(A) Dura was collected from consecutive, severe TBI patients undergoing decompressive craniectomy to alleviate elevated intracranial pressure. ILCs were sorted using forward scatter (FSC)/side scatter (SSC) & identified as CD45+, lineage-negative (Lin−), CD127+ lymphoid cells. ILCs subtypes were further defined as ILC1:, CD45+Lin−CD127+CD161+NKp44+; ILC2, CD45+Lin−CD127+GATA3+CRTH2+; & ILC3, CD45+Lin−CD127+ROR gammat+AhR+, as shown in representative flow cytometry scatterplots. Gray shaded areas indicate isotype controls. To demonstrate functionality, ILCs were further stimulated with cytokine cocktails, & production of signature cytokines was assessed (ILC1, IFN-gamma ; ILC2, IL-5/IL-13; ILC3, IL-17). (B & C) Frequency of ILC subtypes from individual patients, expressed as total cell number (B) & % leukocytes (C) (n = 5). Scatterplots depict mean ± SD. (D) Computed tomography scan of a TBI patient before (Pre-) & after (Post-) decompressive craniectomy surgery. The dura was collected during surgery at the time of bone flap removal. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33427206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for AHR Antibody [Alexa Fluor® 700]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: AHR

Aryl hydrocarbon receptor (AHR) is a member of the basic-helix-loop-helix transcription factor protein family that is a normally inactive cytosolic transcription factor. Upon ligand binding AhR translocates to the nucleus and dimerizes with the AhR nuclear translocator (ARNT), leading to gene transcription alterations.

AHR is expressed in all tissues and most likely plays an important role in the development and maturation of many tissues.

Long Name

Aryl Hydrocarbon Receptor

Alternate Names

BHLHE76

Gene Symbol

AHR

Additional AHR Products

Product Documents for AHR Antibody [Alexa Fluor® 700]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AHR Antibody [Alexa Fluor® 700]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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