We have a customer who will use Cat.No. NB100-2289B for Western Blot of mouse samples. The customer will furthermore try to use the antibody for flow cytometry with mouse samples. Therefore, the customer would like to know if you can estimate if the binding site of the antibody at the c-terminus will be inside the protein when the protein is folded in its natural state or on the outside? Do you have any information if this antibody might be suitable for the use in Flow Cytometry? And do you have any information, maybe from blasting the immunogen, if this antibody cross-reacts with any other mouse proteins? First, I thought the part of Localization on your data sheet refers to the protein AHR itself, not only the immunogen? Could you let me know which aa of the c-terminus of AHR are used as an immunogen for this antibody or at least narrow down the region? Could you let me know if this antibody os IgG or IgM and could you let me know which isotype should be used with this antibody?

Name: AHR Antibody [Biotin]
Brand: Novus Biologicals
SKU: NB100-2289B

Localization is First cytoplasmic, upon binding with ligand and interaction with a HSP90, it translocates to the nucleus. This antibody binds to the cytoplasmic side of the protein. This antibody has never been tested for use in flow cytometry, but since it works in ICC, it should work just fine for flow and is not predicted to cross-react with any other mouse proteins. The immunogen used to generate this polyclonal antibody is a very long peptide that falls between amino acids 400-848 of AHR. As a polyclonal, if will recognize epitopes along this entire region. As a rabbit polyclonal, it is an IgG antibody.

AHR Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2289B

Submit a review

Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB100-2289AF350

View Product

Alexa Fluor 405

NB100-2289AF405

View Product

Alexa Fluor 488

NB100-2289AF488

View Product

Alexa Fluor 532

NB100-2289AF532

View Product

Alexa Fluor 594

NB100-2289AF594

View Product

Alexa Fluor 647

NB100-2289AF647

View Product

Alexa Fluor 700

NB100-2289AF700

View Product

Alexa Fluor 750

NB100-2289AF750

View Product

Allophycocyanin

NB100-2289APC

View Product

CoraFluor 1

NB100-2289CL1

View Product

DyLight 350

NB100-2289UV

View Product

DyLight 405

NB100-2289V

View Product

DyLight 488

NB100-2289G

View Product

DyLight 550

NB100-2289R

View Product

DyLight 594

NB100-2289DL594

View Product

DyLight 650

NB100-2289C

View Product

DyLight 680

NB100-2289FR

View Product

DyLight 755

NB100-2289IR

View Product

FITC

NB100-2289F

View Product

HRP

NB100-2289H

View Product

Janelia Fluor 525

NB100-2289JF525

View Product

Janelia Fluor 549

NB100-2289JF549

View Product

Janelia Fluor 646

NB100-2289JF646

View Product

Janelia Fluor 669

NB100-2289JF669

View Product

mFluor Violet 450 SE

NB100-2289MFV450

View Product

mFluor Violet 500 SE

NB100-2289MFV500

View Product

mFluor Violet 610 SE

NB100-2289MFV610

View Product

NB100-2289PE

View Product

PerCP

NB100-2289PCP

View Product

Forumulation

Catalog #

BSA Free

NB100-2289

View Product

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Guinea Pig

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Simple Western, Western Blot

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AHR Antibodies »

Product Specifications

Immunogen

Bacterially expressed human Aryl hydrocarbon Receptor (C-terminus). [UniProt# P35869]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23887904). Porcine reactivity reported from a verified customer review.

Localization

First cytoplasmic, upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AHR Antibody [Biotin]

Flow Cytometry: AHR Antibody [Biotin] [NB100-2289B] - An intracellular stain was performed on U-87 cells with AHR Antibody NB100-2289B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).