Alkaline Phosphatase/ALPP Antibody (ALPP/516)

Validated:

Unconjugated

Monoclonal Mouse IgG₁ kappa Clone # ALPP/516

0.2 mg/ml

Recombinant full-length human Alkaline Phosphatase/ALPP protein (Uniprot: P05187)

Cytoplasmic and cell surface

PLAP is a tissue specific, trophoblast-derived, 70kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo-glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non-tissue specific AP is found in kidney, liver and bone. This monoclonal antibody binds equally well to all common allelic variants (S, F, FS and I) of PLAP and to some variants of AP from normal human testis. This monoclonal antibody can be used as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes.

Germ Cell Tumor Marker

Monoclonal

Mouse

IgG₁ kappa

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47991)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Protein A or G purified

10 mM PBS with 0.05% BSA

0.05% Sodium Azide

0.2 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C.