AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07764

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # CPTC-CRYAB-1

Concentration

0.2 mg/ml

View all AlphaB Crystallin/CRYAB Antibodies »

Product Summary for AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1)

Immunogen

Recombinant human full-length AlphaB Crystallin/CRYAB protein (Uniprot: P02511)

Localization

Cytoplasm. Translocates to nucleus during heat shock and resides in nuclear splicing speckles.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08380)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1)

Western Blot: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764]

Western Blot: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764]

Western Blot: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Western Blot Analysis of HeLa cell lysate using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Formalin-fixed, paraffin-embedded Rat Heart Muscle stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Formalin-fixed, paraffin-embedded human Breast stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).
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Applications for AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: AlphaB Crystallin/CRYAB

Crystallin AB is a member of the small heat shock protein family and expressed in several tissues including brain, kidney cardiac and skeletal muscle. Crystallin AB functions as a molecular chaperone and in intracellular architecture. Crystallin AB has shown to have interactions with CRYAA, CRYGC, CRYBB2, CRYBA1 and HSPB8. Defects in crystalline AB have been linked to cause myopathy myofibrillar type 2, cataract posterior polar type 2, and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Gene Symbol

CRYAB

UniProt

P02511 (Human)

Additional AlphaB Crystallin/CRYAB Products

Product Documents for AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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