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AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08380

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # CPTC-CRYAB-1

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human full-length AlphaB Crystallin/CRYAB protein (Uniprot: P02511)

Localization

Cytoplasm. Translocates to nucleus during heat shock and resides in nuclear splicing speckles.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07764).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free [NBP3-08380]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free [NBP3-08380]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free [NBP3-08380] - Formalin-fixed, paraffin-embedded Rat Heart Muscle stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).
Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free [NBP3-08380]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free [NBP3-08380]

Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free [NBP3-08380] - Formalin-fixed, paraffin-embedded human Breast stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).
Protein Array: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free [NBP3-08380]

Protein Array: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free [NBP3-08380]

Protein Array: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free [NBP3-08380] - Analysis of Protein Array containing more than 19,000 full-length human proteins using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CRYAB-1).

Applications for AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: AlphaB Crystallin/CRYAB

Crystallin AB is a member of the small heat shock protein family and expressed in several tissues including brain, kidney cardiac and skeletal muscle. Crystallin AB functions as a molecular chaperone and in intracellular architecture. Crystallin AB has shown to have interactions with CRYAA, CRYGC, CRYBB2, CRYBA1 and HSPB8. Defects in crystalline AB have been linked to cause myopathy myofibrillar type 2, cataract posterior polar type 2, and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Gene Symbol

CRYAB

Additional AlphaB Crystallin/CRYAB Products

Product Documents for AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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