Androgen R/NR3C4 [p Ser210] Antibody (156C135.2) [Janelia Fluor® 646]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-56603JF646
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Janelia Fluor 646
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 156C135.2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248. Note: S* refers to phosphorylated serine in the peptide sequence.
Reactivity Notes
Predicted cross-reactivity with Chimpanzee, Canine, Mouse, Porcine and Rat. Mouse reactivity reported in scientific literature (PMID: 21464326).
Specificity
In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this antibody has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for Androgen R/NR3C4 [p Ser210] Antibody (156C135.2) [Janelia Fluor® 646]
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Androgen R/NR3C4
Long Name
Androgen Receptor
Alternate Names
AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1
Gene Symbol
AR
Additional Androgen R/NR3C4 Products
Product Specific Notices for Androgen R/NR3C4 [p Ser210] Antibody (156C135.2) [Janelia Fluor® 646]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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