Androgen R/NR3C4 [p Ser210, p Ser213] Antibody (156C135.2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31099
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Canine, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 156C135.2
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248. Note: S* refers to phosphorylated serine in the peptide sequence.
Reactivity Notes
Predicted cross-reactivity with Chimpanzee, Canine, Mouse, Porcine and Rat.
Modification
p Ser210, p Ser213
Specificity
In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this antibody has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images
Western Blot: Androgen R/NR3C4 [p Ser210, p Ser213] Antibody (156C135.2) - Azide Free [NBP2-31099] - LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h. (See Lin, H-K., Y-C Hu, L. Yang, S. Altuwaijri. 2003 Fig. 2)
Applications
Application
Recommended Usage
Immunohistochemistry
1:20-1:1000
Immunohistochemistry-Paraffin
1:20-1:1000. Use reported in scientific literature (Lin et al (2007))
Western Blot
1-4 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Androgen R/NR3C4
Long Name
Androgen Receptor
Alternate Names
AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1
Entrez Gene IDs
367 (Human)
Gene Symbol
AR
Additional Androgen R/NR3C4 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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