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Angiopoietin-2 Antibody (1F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26509

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26509-100ul
NBP3-26509-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1F10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Angiopoietin-2 Antibody (1F10)

Immunogen

A synthesized peptide derived from Human Angiopoietin-2 [UniProt O15123]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Angiopoietin-2 Antibody (1F10)

Angiopoietin-2 Antibody (1F10)

Immunohistochemistry: Angiopoietin-2 Antibody (1F10) [NBP3-26509] -

Immunohistochemistry: Angiopoietin-2 Antibody (1F10) [NBP3-26509] - Image of Angiopoietin-2 Antibody (1F10) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Angiopoietin-2 Antibody (1F10)

Immunohistochemistry: Angiopoietin-2 Antibody (1F10) [NBP3-26509] -

Immunohistochemistry: Angiopoietin-2 Antibody (1F10) [NBP3-26509] - Image of Angiopoietin-2 Antibody (1F10) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Angiopoietin-2 Antibody (1F10)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Angiopoietin-2

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Alternate Names

ANGPT2

Gene Symbol

ANGPT2

Additional Angiopoietin-2 Products

Product Documents for Angiopoietin-2 Antibody (1F10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Angiopoietin-2 Antibody (1F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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