Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-85467

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Chicken

Cited:

Human, Avian - Chicken

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunofluorescence, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # MM0020-1F29

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Angiopoietin-2 Antibodies »

Product Specifications

Immunogen

Human recombinant Angiopoietin-2

Reactivity Notes

Chicken data from customer review.

Specificity

No cross reactivity with human Angiopoietin-1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Scientific Data Images for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunocytochemistry/ Immunofluorescence: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunocytochemistry/Immunofluorescence: Angiopoietin-2 Antibody (MM0020-1F29) [NB110-85467] - Staining of frozen human skin sections using Angiopoietin 2 antibody at 1:25 dilution. Image provided via product review by verified customer.
Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) [NB110-85467] - Ang-2 (green) was detected in human skin (nevus) using Ang2-Alexa Fluor 488 antibody (1:40) in PBS for 1 hour. Nuclei were stained with DAPI (blue). Tissue was fixed with acetone. Image from a verified customer review. Image using the Alexa Fluor 488 format of this antibody.
Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free [NB110-85467]

Immunohistochemistry: Angiopoietin-2 Antibody (MM0020-1F29) [NB110-85467] - Normal Human placenta tissue

Applications for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:25

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:500-1:1000
Application Notes
By Western blot, this antibody detects a band at ~60-75 kDa under reducing conditions. Has been reported to work in Immunohistochemistry-Frozen (PMID 22302773) and Immunofluorescence in a customer review.

Reviewed Applications

Read 2 reviews rated 3 using NB110-85467 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Angiopoietin-2

Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2/Tek. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin 1 binding to Tie 2/Tek causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Alternate Names

ANGPT2

Entrez Gene IDs

285 (Human)

Gene Symbol

ANGPT2

UniProt

O15123

Additional Angiopoietin-2 Products

Product Documents for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Angiopoietin-2 Antibody (MM0020-1F29) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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