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Anti- Daclizumab (Anti-Idiotype) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10218

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB10218-100
MAB10218-SP

Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2498A

Product Specifications

Immunogen

Full length Daclizumab

Specificity

Detects Daclizumab in direct ELISAs and Western blots. In Western blots, no cross-reactivity with Basiliximab, Satumomab, Teplizumab, and Alemtuzumab is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anti- Daclizumab (Anti-Idiotype) Antibody

Detection of Daclizumab antibody by Western Blot.

Detection of Daclizumab by Western Blot.

Western blot shows Daclizumab, Basiliximab (negative control), Satumomab (negative control), Teplizumab (negative control), and Alemtuzumab (negative control) biosimilars. PVDF membrane was probed with 2 µg/mL of Anti- Daclizumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB10218) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Daclizumab at approximately 250 kDa (as indicated). Human IgG is shown as a loading control. This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Applications for Anti- Daclizumab (Anti-Idiotype) Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Daclizumab
under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Daclizumab

The anti-Daclizumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Daclizumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Additional Daclizumab Products

Product Documents for Anti- Daclizumab (Anti-Idiotype) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Anti- Daclizumab (Anti-Idiotype) Antibody

For research use only

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