Detects Rituximab in Western blots in direct ELISAs. 100% cross-reactivity with Tositumomab and Ibritumomab (95% or higher sequence identity with Rituximab) and no cross-reactivity with Cetuximab, Trastuzumab, Brentuximab, Gemtuzumab, Adalimumab, Solanezumab, Teprotumumab, and Aducanumab is observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Anti- Rituximab (Anti-Idiotype) PE-conjugated Antibody

Detection of Rituximab in PBMCs with CD19 costain cells by Flow Cytometry.

PBMCs with CD19 costain were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Rabbit Anti-Rituximab (Anti-Idiotype) PE-conjugated Monoclonal Antibody (Catalog # FAB9630P) or (B) Rabbit IgG PE-conjugated Antibody (Catalog # IC1051P). View our protocol for Staining Membrane-associated Proteins.

Applications for Anti- Rituximab (Anti-Idiotype) PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: PBMCs with CD19 costain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitution default not known

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Rituximab

The anti-Rituximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Rituximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats. In addition to Rituximab, the anti-Idiotype antibody also detects derivatives of Rituximab (Ibritumumab and Tositumumab).

Alternate Names

B lymphocyte antigen CD20, B lymphocyte surface antigen B1, Bp35, CD20, LEU 16, LEU16, Membrane spanning 4 domains subfamily A member 1, MS4A1

Additional Rituximab Products

Product Documents for Anti- Rituximab (Anti-Idiotype) PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Anti- Rituximab (Anti-Idiotype) PE-conjugated Antibody

For research use only

Citations
Reviews

Calculators
Spectra Viewer
Flow Cytometry

Anti- Rituximab (Anti-Idiotype) PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9630P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Rituximab Antibodies »

Product Specifications

Immunogen

Specificity