Apc4 Antibody (CIV1.1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50105
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # CIV1.1
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
C-terminus of Apc4
Specificity
CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution. Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently.Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications for Apc4 Antibody (CIV1.1) - BSA Free
Application
Recommended Usage
Immunoprecipitation
1:10 - 1:500
Western Blot
1:500
Application Notes
WB - It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently. Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an Apc4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.Positive control(s): Purified APC/C by silver and mass spectrometry.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Apc4
Alternate Names
anaphase promoting complex subunit 4, anaphase-promoting complex subunit 4, APC4Cyclosome subunit 4
Gene Symbol
ANAPC4
Additional Apc4 Products
Product Documents for Apc4 Antibody (CIV1.1) - BSA Free
Product Specific Notices for Apc4 Antibody (CIV1.1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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