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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Mouse IgG Clone # CIV1.1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

C-terminus of Apc4

Specificity

CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution. Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently.Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Applications for Apc4 Antibody (CIV1.1) [Alexa Fluor® 594]

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Apc4

APC4 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase that controls cell cycle progression and promotes the transition from metaphase to anaphase. The APC complex is composed of at least 11 APC components. The exact function of APC4 has not been determined.

Alternate Names

anaphase promoting complex subunit 4, anaphase-promoting complex subunit 4, APC4Cyclosome subunit 4

Gene Symbol

ANAPC4

Additional Apc4 Products

Product Documents for Apc4 Antibody (CIV1.1) [Alexa Fluor® 594]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apc4 Antibody (CIV1.1) [Alexa Fluor® 594]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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