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Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free Best Seller

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60531

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat (Negative)

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # WUE-4

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Purified human ApoE [UniProt# P02649]

Epitope

Residues 140-160 of purified human ApoE (LDLR receptor binding site).

Reactivity Notes

Reacts with human better than mouse. It does not appear to react with rat brain tissue.

Localization

Secreted

Specificity

This antibody detects ApoE2, ApoE3 and ApoE4 (PMID 2466929).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4)BSA Free [NB110-60531]

Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4)BSA Free [NB110-60531]

Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain
Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free [NB110-60531]

Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Applications for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:10-1:500

Western Blot

2 ug/ml
Application Notes
This ApoE antibody is useful for Western blot, ELISA, Immunohistochemistry and Immunoprecipitation. In Western blot a band is observed at ~36 kDa, representing the ApoE protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26564908). This antibody is CyTOF ready.

Reviewed Applications

Read 2 reviews rated 4.5 using NB110-60531 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein E/ApoE

ApoE (apolipoprotein E) is the protein constituent of cholesterol/triglyceride-rich plasma lipoproteins, and is a multifunctional glycosylated secretary protein found almost in all organs with high activity in hepatic tissues. ApoE expression is induced by cholesterol-rich diets and is enhanced in lipoproteins in humans with genetic disorder type III hyperlipoproteinemia (HLP) characterized by remnant lipoproteins accumulation in plasma and premature atherosclerosis. ApoE circulates in blood as a protein component of VLDLs, chylomicron remnants, a subclass of HDL etc, and in cerebrospinal fluid as well as CNS interstitial fluid on small particles and disks resembling HDLs. ApoE facilitates transport of cholesterol and other lipids, as well as the clearance of plasma lipoproteins by serving as a critical ligand for lipoprotein uptake by LDL receptors and related proteins. ApoE participates in lipids redistribution to cells (e.g. CNS) that require cholesterol and phospholipids for reparative processes. ApoE also involves in proliferation inhibition of smooth muscle cells/lymphocytes, antigen presentation, and cholesterol efflux stimulation from foam cell macrophages. Defects in APOE have been linked to HLPP3 (hyperlipoproteinemia type 3), AD2 (Alzheimer disease type 2), SBHD (sea-blue histiocyte disease), LPG (lipoprotein glomerulopathy), and certain autoimmune disorders including multiple sclerosis and psoriasis.

Alternate Names

APOE

Gene Symbol

APOE

Additional Apolipoprotein E/ApoE Products

Product Documents for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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