Axin-1 Antibody (4E9F1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61695

Novus Biologicals, part of Bio-Techne
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NBP2-61695
NBP2-61695-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 4E9F1

Format

BSA Free

Concentration

1 mg/ml

View all Axin-1 Antibodies »

Product Summary for Axin-1 Antibody (4E9F1) - BSA Free

Immunogen

Purified recombinant fragment of human Axin-1 (AA: 546-752) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

95.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Axin-1 Antibody (4E9F1) - BSA Free

Western Blot: Axin-1 Antibody (4E9F1)BSA Free [NBP2-61695]

Western Blot: Axin-1 Antibody (4E9F1)BSA Free [NBP2-61695]

Western Blot: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis using AXIN1 mAb against human AXIN1 (AA: 546-752) recombinant protein. (Expected MW is 48.7 kDa)
Immunocytochemistry/ Immunofluorescence: Axin-1 Antibody (4E9F1) - BSA Free [NBP2-61695]

Immunocytochemistry/ Immunofluorescence: Axin-1 Antibody (4E9F1) - BSA Free [NBP2-61695]

Immunocytochemistry/Immunofluorescence: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis of Hela cells using AXIN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Flow Cytometry: Axin-1 Antibody (4E9F1) - BSA Free [NBP2-61695]

Flow Cytometry: Axin-1 Antibody (4E9F1) - BSA Free [NBP2-61695]

Flow Cytometry: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis of Hela cells using AXIN1 mouse mAb (green) and negative control (red).
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Applications for Axin-1 Antibody (4E9F1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:50-1:250

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Axin-1

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants.

Long Name

Axis Inhibitor 1

Alternate Names

Axin1, MGC52315

Gene Symbol

AXIN1

Additional Axin-1 Products

Product Documents for Axin-1 Antibody (4E9F1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Axin-1 Antibody (4E9F1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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