Axin-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1011
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Summary for Axin-1 Antibody
Immunogen
Peptide with sequence C-FEEKIIGKVEKVD corresponding to C-Terminus according to NP_851393.1.
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.
Specificity
This antibody is expected to recognise both reported isoforms.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Axin-1 Antibody
![Immunohistochemistry: Axin-1 Antibody [NB100-1011]](https://resources.bio-techne.com/images/products/Axin-1-Antibody-Immunohistochemistry-NB100-1011-img0002.jpg "Immunohistochemistry: Axin-1 Antibody [NB100-1011]")
Immunohistochemistry: Axin-1 Antibody [NB100-1011]
Immunohistochemistry: Axin-1 Antibody [NB100-1011] - (3.8ug/ml) staining of paraffin embedded Human Adrenal Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Axin-1 Antibody
Application
Recommended Usage
Immunohistochemistry
3 - 5 ug/ml
Immunohistochemistry-Paraffin
3 - 5 ug/ml
Peptide ELISA
Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave bands at approx. 150 kDa band and 22-24 kDa band in human brain (hippocampus) lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.6 kDa band according to NP_003493.1 and 91.7 kDa band according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human adrenal gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Axin-1
Long Name
Axis Inhibitor 1
Alternate Names
Axin1, MGC52315
Gene Symbol
AXIN1
UniProt
Additional Axin-1 Products
Product Documents for Axin-1 Antibody
Product Specific Notices for Axin-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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