Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Axin-1 Antibodies »

Product Summary for Axin-1 Antibody

Immunogen

Synthetic peptide of Human Axin-1 (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).

Localization

Cytoplasm|Nucleus|Membrane|Cell Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Axin-1 Antibody

Western Blot: Axin-1 Antibody [NBP3-18242]

Western Blot: Axin-1 Antibody [NBP3-18242]

Western Blot: Axin-1 Antibody [NBP3-18242] - Western blot analysis of Mouse kidney lysate showing detection of ~95.6 kDa Axin-1 protein using Rabbit Anti-Axin-1 Polyclonal Antibody (NBP3-18242). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse kidney lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-1 Polyclonal Antibody (NBP3-18242) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~95.6 kDa.

Applications for Axin-1 Antibody

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS pH 7.4, 50% glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Axin-1

Negative regulator of Wnt signaling. Also associated with cancer.

Long Name

Axis Inhibitor 1

Alternate Names

Axin1, MGC52315

Gene Symbol

AXIN1

Additional Axin-1 Products

Product Documents for Axin-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Axin-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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