Axin-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25292
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Axin-1 Antibody
Immunogen
This antibody has been engineered to specifically recognize the recombinant protein Axin-1 using the following amino acid sequence: AWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGAASGHGKHVPKS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Axin-1 Antibody
![Axin-1 Antibody Western Blot: Axin-1 Antibody [NBP3-25292]](https://resources.bio-techne.com/images/products/nbp3-25292_rabbit-axin-1-pab-2012202314593078.jpg "Western Blot: Axin-1 Antibody [NBP3-25292]")
Western Blot: Axin-1 Antibody [NBP3-25292]
Lane 1: Marker [kDa] 250,130,95,72,55,36,28,17,10Lane 2: RT-4
Lane 3: U-251MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
![Axin-1 Antibody Immunocytochemistry/Immunofluorescence: Axin-1 Antibody [NBP3-25292]](https://resources.bio-techne.com/images/products/nbp3-25292_rabbit-axin-1-pab-2012202314593056.jpg "Immunocytochemistry/Immunofluorescence: Axin-1 Antibody [NBP3-25292]")
Immunocytochemistry/Immunofluorescence: Axin-1 Antibody [NBP3-25292]
Staining of human cell line SiHa shows localization to nucleoli.Applications for Axin-1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 µg/ml
Western Blot
0.04-0.4 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Axin-1
Long Name
Axis Inhibitor 1
Alternate Names
Axin1, MGC52315
Gene Symbol
AXIN1
Additional Axin-1 Products
Product Documents for Axin-1 Antibody
Product Specific Notices for Axin-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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