B-Raf Antibody (CL4004)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59035

Novus Biologicals, part of Bio-Techne
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NBP2-59035
NBP2-59035-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL4004

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all B-Raf Antibodies »

Product Summary for B-Raf Antibody (CL4004)

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:PIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSS

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for B-Raf Antibody (CL4004)

Western Blot: B-Raf Antibody (CL4004) [NBP2-59035]

Western Blot: B-Raf Antibody (CL4004) [NBP2-59035]

Western Blot: B-Raf Antibody (CL4004) [NBP2-59035] - Analysis in human cell line MOLT-4.
Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035]

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035]

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human testis shows cytoplasmic positivity in seminiferous tubules.
Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035]

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035]

Immunohistochemistry-Paraffin: B-Raf Antibody (CL4004) [NBP2-59035] - Staining of human prostate cancer shows moderate cytoplasmic immunoreactivity in tumor cells.
View 2 more images

Applications for B-Raf Antibody (CL4004)

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf Antibody (CL4004)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B-Raf Antibody (CL4004)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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