B-Raf Antibody (CL4004) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44118

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-44118
Print Quote
Bulk Order
Forumulation
Catalog #
BSA Free
NBP2-59035
View Product
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL4004

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all B-Raf Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P15056, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for B-Raf Antibody (CL4004) - Azide and BSA Free

B-Raf Antibody (CL4004) - Azide and BSA Free Immunohistochemistry-Paraffin: B-Raf Antibody [NBP3-44118]

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP3-44118]

Staining of human breast cancer shows moderate cytoplasmic immunoreactivity in tumor cells.
B-Raf Antibody (CL4004) - Azide and BSA Free Immunohistochemistry-Paraffin: B-Raf Antibody [NBP3-44118]

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP3-44118]

Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
B-Raf Antibody (CL4004) - Azide and BSA Free Immunohistochemistry-Paraffin: B-Raf Antibody [NBP3-44118]

Immunohistochemistry-Paraffin: B-Raf Antibody [NBP3-44118]

Staining of human prostate cancer shows moderate cytoplasmic immunoreactivity in tumor cells.
View 2 more images

Applications for B-Raf Antibody (CL4004) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf Antibody (CL4004) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for B-Raf Antibody (CL4004) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.