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B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75194

Novus Biologicals, part of Bio-Techne
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NBP2-75194
NBP2-75194-0.025mg

Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 kappa Clone # MJ18

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1-242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.

Specificity

This antibody binds to mouse B7-H3 and does not react with other B7-family molecules.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Scientific Data Images for B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free

Immunofluorescence: B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free [NBP2-75194]

Immunofluorescence: B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free [NBP2-75194]

Immunofluorescence: B7-H3/CD276 Antibody (MJ18) [NBP2-75194] - Staining of fixed mouse splenocytes with anti-B7-H3/CD276 antibody MJ18.
ELISA: B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free [NBP2-75194]

ELISA: B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free [NBP2-75194]

ELISA: B7-H3/CD276 Antibody (MJ18) [NBP2-75194] - Blue line: Isotype control. Orange line: NBP2-75194.

Applications for B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: B7-H3

Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).

Long Name

B7 Homolog 3

Alternate Names

B7H3, CD276

Gene Symbol

CD276

Additional B7-H3 Products

Product Documents for B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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