B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75194
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 kappa Clone # MJ18
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free
Immunogen
This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1-242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.
Specificity
This antibody binds to mouse B7-H3 and does not react with other B7-family molecules.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1 kappa
Scientific Data Images for B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free
![Immunofluorescence: B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free [NBP2-75194]](https://resources.bio-techne.com/images/products/B7-H3-CD276-Antibody-MJ18-Immunofluorescence-NBP2-75194-img0001.jpg "Immunofluorescence: B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free [NBP2-75194]")
Immunofluorescence: B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free [NBP2-75194]
Immunofluorescence: B7-H3/CD276 Antibody (MJ18) [NBP2-75194] - Staining of fixed mouse splenocytes with anti-B7-H3/CD276 antibody MJ18.![ELISA: B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free [NBP2-75194]](https://resources.bio-techne.com/images/products/B7-H3-CD276-Antibody-MJ18-ELISA-NBP2-75194-img0003.jpg "ELISA: B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free [NBP2-75194]")
ELISA: B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free [NBP2-75194]
ELISA: B7-H3/CD276 Antibody (MJ18) [NBP2-75194] - Blue line: Isotype control. Orange line: NBP2-75194.Applications for B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: B7-H3
Long Name
B7 Homolog 3
Alternate Names
B7H3, CD276
Gene Symbol
CD276
Additional B7-H3 Products
Product Documents for B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free
Product Specific Notices for B7-H3/CD276 Antibody (MJ18) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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