B7-H3/CD276 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88965

Novus Biologicals, part of Bio-Techne
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NBP1-88965
NBP1-88965-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all B7-H3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for B7-H3/CD276 Antibody

Knockout Validated: B7-H3/CD276 Antibody [NBP1-88965]

Knockout Validated: B7-H3/CD276 Antibody [NBP1-88965]

Knockout Validated: B7-H3/CD276 Antibody [NBP1-88965] - Western blot shows lysates of U2OS human bone osteosarcoma parental cell line and B7-H3 knockout (KO) U2OS cell line. PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human B7-H3 Polyclonal Antibody (Catalog # NBP1-88965) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for B7-H3 at approximately 95 kDa (as indicated) in the parental U2OS cell line, but is not detectable in the knockout U2OS cell line. This experiment was conducted under reducing conditions.
Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965]

Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965]

Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP1-88965] - Staining in human prostate and pancreas tissues using anti-CD276 antibody. Corresponding CD276 RNA-seq data are presented for the same tissues.
Western Blot: B7-H3/CD276 Antibody [NBP1-88965]

Western Blot: B7-H3/CD276 Antibody [NBP1-88965]

Western Blot: B7-H3/CD276 Antibody [NBP1-88965] - Analysis in control (vector only transfected HEK293T lysate) and CD276 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Applications for B7-H3/CD276 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B7-H3

Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).

Long Name

B7 Homolog 3

Alternate Names

B7H3, CD276

Gene Symbol

CD276

Additional B7-H3 Products

Product Documents for B7-H3/CD276 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B7-H3/CD276 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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