BAP1 Antibody (1G8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80589

Novus Biologicals, part of Bio-Techne
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NBP2-80589
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G8

Format

Azide and BSA Free

Concentration

1 mg/ml

View all BAP1 Antibodies »

Product Specifications

Immunogen

Full-length human BAP1 protein expressed and purified from E.coli. [UniProt# Q92560]

Epitope

Within the UCH domain of BAP1.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BAP1 Antibody (1G8) - Azide and BSA Free

Western Blot: BAP1 Antibody (1G8)Azide and BSA Free [NBP2-80589]

Western Blot: BAP1 Antibody (1G8)Azide and BSA Free [NBP2-80589]

Western Blot: BAP1 Antibody (1G8) - Azide and BSA Free [NBP2-80589] - Detection of BAP1 in HeLa WCE. Image from the standard format of this antibody.
Flow Cytometry: BAP1 Antibody (1G8) - Azide and BSA Free [NBP2-80589]

Flow Cytometry: BAP1 Antibody (1G8) - Azide and BSA Free [NBP2-80589]

Flow Cytometry: BAP1 Antibody (1G8) - Azide and BSA Free [NBP2-80589] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using BAP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.

Applications for BAP1 Antibody (1G8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Western Blot

2 ug/ml
Application Notes
This BAP1 antibody is useful for Western blot. In Western blot a band is seen at ~85 kDa, representing the BAP1 protein. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAP1

BAP1 (BRCA1-associated protein 1) was initially identified as BRCA1 binding protein and subsequently it was found to be a nuclear deubiquitinating enzyme belonging to ubiquitin C-terminal hydrolase family that play a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. BAP1 forms the catalytic component of PR-DUB complex (BAP1 and ASXL1) that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). BAP1 regulates cell growth by mediating deubiquitination of HCFC1 N- and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. BAP1 interferes with activity of BRCA1-BARD1 heterodimer by inhibiting their ability to mediate ubiquitination/autoubiquitination. BAP1 is considered a tumour suppressor and defects in BAP1 gene have been linked to tumor predisposition syndrome (TPDS). Germline mutations of BAP1 confers increased susceptibility for certain malignancies including uveal melanoma, epithelioid atypical Spitz tumours, cutaneous melanoma, and mesothelioma. Somatic BAP1 mutations are linked to cutaneous melanocytic tumours, uveal melanoma, mesothelioma, clear cell renal cell carcinoma etc.

Long Name

BRCA1-associated protein 1

Alternate Names

Cerebral Protein-6, UCHL2

Gene Symbol

BAP1

Additional BAP1 Products

Product Documents for BAP1 Antibody (1G8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BAP1 Antibody (1G8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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