Bcl-10 Antibody (BL10/411)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29422

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # BL10/411

Concentration

0.2 mg/ml

View all Bcl-10 Antibodies »

Product Summary for Bcl-10 Antibody (BL10/411)

Immunogen

Human Bcl-10 recombinant protein (epitope aa122-168) (Uniprot: O95999)

Localization

Nuclear and cytoplasmic

Marker

MALT-Lymphoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33190)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Bcl-10 Antibody (BL10/411)

Western Blot: Bcl-10 Antibody (BL10/411) [NBP2-29422]

Western Blot: Bcl-10 Antibody (BL10/411) [NBP2-29422]

Western Blot: Bcl-10 Antibody (BL10/411) [NBP2-29422] - HepG2 Cell Lysate using Bcl-10 Monoclonal Antibody (BL10/411).
Immunohistochemistry-Paraffin: Bcl-10 Antibody (BL10/411) [NBP2-29422]

Immunohistochemistry-Paraffin: Bcl-10 Antibody (BL10/411) [NBP2-29422]

Immunohistochemistry-Paraffin: Bcl-10 Antibody (BL10/411) [NBP2-29422] - Formalin-paraffin human tonsil stained with Bcl-10 Ab (BL10/411).
Flow Cytometry: Bcl-10 Antibody (BL10/411) [NBP2-29422]

Flow Cytometry: Bcl-10 Antibody (BL10/411) [NBP2-29422]

Flow Cytometry: Bcl-10 Antibody (BL10/411) [NBP2-29422] - Flow Cytometric Analysis of PFA-fixed K562 cells using Bcl-10 Antibody (BL10/411) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Applications for Bcl-10 Antibody (BL10/411)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Bcl-10

Bcl-10, also called CIPER, c-CARMEN and mE10, was first identified as a gene truncated or mutated in MALT B cell lymphomas and other tumor types.

Long Name

B Cell Lymphoma/Leukemia 10

Alternate Names

Bcl10, c-E10, CARMEN, CIPER, CLAP, mE10

Gene Symbol

BCL10

UniProt

O95999

Additional Bcl-10 Products

Product Documents for Bcl-10 Antibody (BL10/411)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Bcl-10 Antibody (BL10/411)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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