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Bcl-10 Antibody (SPM520)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34368

Novus Biologicals, part of Bio-Techne
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NBP2-34368-0.02mg
NBP2-34368-0.1mg

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM520

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Human Bcl-10 recombinant protein (epitope aa122-168) (Uniprot: O95999)

Localization

Nuclear and cytoplasmic

Marker

MALT-Lymphoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34415)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Bcl-10 Antibody (SPM520)

Immunohistochemistry-Paraffin: Bcl-10 Antibody (SPM520) [NBP2-34368]

Immunohistochemistry-Paraffin: Bcl-10 Antibody (SPM520) [NBP2-34368]

Immunohistochemistry-Paraffin: Bcl-10 Antibody (SPM520) [NBP2-34368] - Formalin-fixed, paraffin-embedded human Tonsil stained with BCL10 Monoclonal Antibody (SPM250).

Applications for Bcl-10 Antibody (SPM520)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Bcl-10

Bcl-10 is an adaptor protein with a caspase recruitment domain (CARD) that promotes apoptosis and activates pro-caspase 9 and NF-kB (1-2). Bcl-10 also interacts with other CARD domain containing proteins including CARD9, 10, 11 and 14. Bcl-10 is found to form a complex with MALT1, a protein known to be translocated in MALT lymphomas. Bcl-10 and MALT1 play a critical role in antigen receptor

Long Name

B Cell Lymphoma/Leukemia 10

Alternate Names

Bcl10, c-E10, CARMEN, CIPER, CLAP, mE10

Gene Symbol

BCL10

UniProt

Additional Bcl-10 Products

Product Documents for Bcl-10 Antibody (SPM520)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Bcl-10 Antibody (SPM520)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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