bcl-x Antibody (2H12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32917
![Western Blot: bcl-x Antibody (2H12) [NBP2-32917]](https://resources.bio-techne.com/images/products/Bcl-xL-Antibody-2H12-Western-Blot-NBP2-32917-img0004.jpg "Western Blot: bcl-x Antibody (2H12) [NBP2-32917]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2H12
Concentration
0.2 mg/ml
Product Specifications
Immunogen
A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-xL protein (Uniprot: Q07817)
Localization
Cytoplasmic and cell/nuclear membrane
Specificity
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This monoclonal antibody reacts with both Bcl-XS and Bcl-XL proteins.
Marker
Apoptosis Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34531)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for bcl-x Antibody (2H12)
Western Blot: bcl-x Antibody (2H12) [NBP2-32917]
Western Blot: bcl-x Antibody (2H12) [NBP2-32917] - Rat and mouse neonatal cardiomyocytes. Western blot image submitted by a verified customer review.![Immunohistochemistry-Paraffin: bcl-x Antibody (2H12) [NBP2-32917]](https://resources.bio-techne.com/images/products/Bcl-xL-Antibody-2H12-Immunohistochemistry-Paraffin-NBP2-32917-img0001.jpg "Immunohistochemistry-Paraffin: bcl-x Antibody (2H12) [NBP2-32917]")
Immunohistochemistry-Paraffin: bcl-x Antibody (2H12) [NBP2-32917]
Immunohistochemistry-Paraffin: bcl-x Antibody (2H12) [NBP2-32917] - FFPE human Hodgkin's lymphoma stained with bcl-x Ab (2H12). Note cytoplasmic and membrane staining.![Western Blot: bcl-x Antibody (2H12) [NBP2-32917]](https://resources.bio-techne.com/images/products/Bcl-xL-Antibody-2H12-Western-Blot-NBP2-32917-img0003.jpg "Western Blot: bcl-x Antibody (2H12) [NBP2-32917]")
Western Blot: bcl-x Antibody (2H12) [NBP2-32917]
Western Blot: bcl-x Antibody (2H12) [NBP2-32917] - Western blot analysis of Jurkat Cell lysate using bcl-X Ab (2H12).Applications for bcl-x Antibody (2H12)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Reviewed Applications
Read 2 reviews rated 5 using NBP2-32917 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Bcl-x
Additional Bcl-x Products
Product Documents for bcl-x Antibody (2H12)
Product Specific Notices for bcl-x Antibody (2H12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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